相关实验视频

Updated: May 10, 2025

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Published on: August 14, 2019

具有抗血栓活性的N-乙化三环碳醇的生物合成

Jiaming Luo¹, Yaoyao Shen¹, Ke Liu²

¹Research Center for Marine Drugs, Department of Pharmacy, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127 People's Republic of China.

Journal of natural products

|April 22, 2025

概括

研究人员设计了一条微生物通路,以产生新的三环碳素类化合物. 一种化合物 - - 碳醇5 - - 在斑马鱼中表现出显著的抗血栓活性,突出了潜在的治疗应用.

科学领域:

自然产品生物合成自然产品生物合成
微生物工程微生物工程
药理学药理学是指药理学的学科.

背景情况:

三环碳素是具有多样化的生物活动的重要药解体.
了解它们的生物合成途径对于发现新的类似物至关重要.

研究的目的:

在*Streptomyces albus*中异质地表达碳-3,4-子生物合成途径.
为了识别和描述由这种工程途径产生的新型碳醇衍生物.
评估分离化合物的生物活性.

主要方法:

在 *Streptomyces albus* J1074.4 中生物合成基因集群的异质表达.
使用高分辨率电喷射电离化质谱 (HR-ESI-MS),核磁共振 (NMR) 和X射线晶体学阐明结构.
酶合成和突变遗传学分析.
使用斑马鱼模型进行生物活性查.

主要成果:

生产一种已知的正基碳醇 (2) 和三个新的N-乙化碳醇 (3-5).
酶合成证实了化合物2作为前体的作用.
鉴定一种阿里胺N-乙转移酶基因,该基因对于生产3-5化合物至关重要.
化合物5在斑马鱼中表现出显著的抗血栓活性.

结论:

这种工程途径成功地产生了多种多样化的三环碳素类化合物.
这项研究揭示了关键的酶和基因元素,涉及到炭醇生物合成.
化合物5显示了抗血栓剂应用的治疗潜力,需要进一步研究.

更多相关视频

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

相关概念视频

What is Photosynthesis?

What is Photosynthesis?

Photosynthesis is a multipart, biochemical process that occurs in plants as well as in some bacteria. It captures carbon dioxide and solar energy to produce glucose. Glucose stores chemical energy in the form of carbohydrates. The overall biochemical formula of photosynthesis is 6 CO2 + 6 H2O + Light energy → C6H12O6 + 6 O2. Photosynthesis releases oxygen into the atmosphere and is largely responsible for maintaining the Earth’s atmospheric oxygen content.

The Calvin Cycle

The Calvin Cycle

The Calvin Benson Cycle

The Calvin Benson Cycle

Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...

Synthetic Biology

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...

What is Metabolism?

What is Metabolism?

Overview of Fatty Acid Metabolism

Overview of Fatty Acid Metabolism

Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...