在温度介导的甲状腺激素诱导的转变过程中,Rana [Lithobates] catesbeiana 组织中的基因组丰度和翻译后修饰的变化
Haley N Kuecks-Winger1, Anita A Thambirajah1, Lorissa M Corrie1
1Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada.
概括
在寒冷的温度下暴露于甲状腺激素 (TH) 会在鱼中建立分子记忆,为更快的转化做准备. 这项研究揭示了温度和组织特异性的表观遗传变化,包括与TH信号和记忆形成相关的基因组修饰.
科学领域:
- 发展生物学 发展生物学
- 内分泌学 在内分泌学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 两变形是一种依赖甲状腺激素 (TH) 的过程,涉及显著的组织重塑.
- 变形取决于温度,TH处理在5°C时无效.
- 在5°C的TH暴露会诱导分子记忆,在允许的温度 (24°C) 下加速后续的转化.
研究的目的:
- 为了研究基因组转化后修饰 (PTM) 和基因组变异在TH诱导的分子记忆中的作用.
- 在不同温度条件下 (24°C,5°C和5°C→24°C) 分析对TH治疗的反应中,基因组组成和PTM的变化.
- 为了识别Rana [Lithobates]catesbeiana的血液,肝脏和尾中的特定组织表观遗传变化.
主要方法:
- 变形前的Rana [Lithobates] catesbeiana头在24°C,5°C或5°C时接受了TH治疗,然后转移到24°C.
- 质谱法用于分析血液,肝脏和尾组织中的基因组合和PTM.
- 使用严格的选择标准来确定染色质结合蛋白的显著变化,包括基因组和相关的PTMs.
主要成果:
- 在允许温度 (24°C) 的TH治疗后,链接组合素变异H1.0在所有组织中增加.
- 甲基-CpG结合蛋白2 (MeCP2) 和高流动性组 (HMG) 蛋白中的TH-依赖性改变是组织和温度特异的.
- 观察到肝脏中H3K27和H3K36甲基化的丰度差异,以及新发现的HMGB2甲基化标记,显示出温度特异性的变化.
结论:
- 表观遗传变异,包括改变的基因组合和PTM,与两变形期间的TH信号相关.
- 这些表观遗传变化是特定于组织和温度的,这表明在冷TH暴露后观察到的分子记忆的建立中发挥了作用.
- 这些发现表明,表观遗传机制可能有助于TH信号的早期事件和的分子记忆的形成.
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