通过酸盐调制,增强Megasphaera hexanoica在含乳酸盐的废水中的酸盐生产
Zeyu Wang1, Jun Chen2, María C Veiga3
1Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Interdisciplinary Research Academy, Zhejiang Shuren University, Hangzhou, 310015, China; Chemical Engineering Laboratory, Faculty of Sciences and Interdisciplinary Centre of Chemistry and Biology - Centro Interdisciplinar de Química y Biología (CICA), BIOENGIN group, University of La Coruña (UDC), E-15008, La Coruña, Spain.
Journal of environmental management
|April 22, 2025
概括
巨花 (Megasphaera hexanoica) 通过反向β-氧化有效地产生酸. 将酸盐度优化至30mM显著提高了菜的产量和生产力,为工业应用铺平了道路.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 代谢工程是代谢工程.
背景情况:
- 麦加斯菲拉 (Megasphaera hexanoica) 是一种无氧细菌,有可能生产鱼.
- 反向β-氧化是卡酸生物合成的关键代谢途径.
- 了解基质利用对于优化微生物生产过程至关重要.
研究的目的:
- 调查酸盐度对M. hexanoica. 酸盐产生的影响.
- 在不同酸盐水平下评估乳酸氧化,细胞合成和酸产量.
- 阐明酸盐生物合成的机械基础和模型的动力学.
主要方法:
- 用不同度的乙酸盐 (10-50毫米) 培养M. hexanoica.
- 料批发发酵和生物反应器测试以评估连续生产.
- 酶活性测定和运动建模 (Logistic和Fitzhugh模型).
主要成果:
- 30毫米的最佳酸盐度产生了42.83毫米的酸盐,电子效率为67.03%.
- 料批量和生物反应器研究实现了持续的鱼产量高达65.25 mM.
- 30毫米酸盐优化了乙烯基-CoA流量和烯基-CoA转移酶活性 (3.5 U/mg).
- 动力模型准确地描述了乳酸消耗 (R2=0.991) 和鱼产量 (R2=0.991).
结论:
- 乙酸度是优化M. hexanoica. caproate生物合成的关键因素.
- 该研究为工业规模的鱼生产提供了机械的理解和动力模型.
- 这项研究提供了增强鱼产量和生产力的实际策略.
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