用生物纳米颗粒进行预防性补充,通过抑制表皮-免疫交叉与肠在芯片上的交叉来缓解肠壁氧化损伤
Lei Qiao1, Ge Yang2, Tianjing Deng2
1School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China; Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Journal of advanced research
|April 22, 2025
概括
生物纳米粒子 (SeNPs) 通过增强抗氧化防御和减少炎症来保护肠道屏障功能. 这些纳米粒子被肠道细胞吸收并纳入抗氧化酶,有效地减轻氧化应激.
科学领域:
- 纳米技术纳米技术
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
背景情况:
- 生物纳米粒子 (SeNPs) 具有显著的生物活性,调节氧化还原和免疫反应.
- 它们独特的物理化学性质使得它们对生物应用具有前景.
研究的目的:
- 阐明肠道上皮细胞对SeNPs的吸收和代谢.
- 通过肠上芯片模型研究SeNPs对肠道屏障功能的保护作用.
- 探索SeNP在氧化应激和免疫平衡中的调节作用.
主要方法:
- 开发一个模仿肠道特征的肠子芯片模型.
- 研究氧化应激对肠道上皮细胞和屏障功能的影响.
- 使用小鼠和小猪模型进行验证.
主要成果:
- 肠上皮细胞 (IPEC-J2) 通过内分泌酶内化SeNPs,并将其代谢成单素,增强抗氧化剂单酶的合成.
- 迪卡特诱导的氧化应激损害了肠道屏障的完整性,并激活了巨细胞,释放了促炎细胞因子 (IL-1β,TNF-α).
- 治疗SeNPs增强了细胞含量,增加了抗氧化酶活性,调节了关键信号通路 (AMPK/NLRP3/Nrf2),减轻了氧化应激,并防止了屏障损伤.
结论:
- 生物性SeNP增强抗氧化防御,并维持线粒体平衡.
- 红细胞核蛋白抑制免疫和上皮细胞之间的炎症交叉.
- SeNP有效地保护肠道上皮屏障免受氧化应激损伤.
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