在灭绝过程中,对实体的内膜投射限制了保护性行为,从而学习灭绝
Carolina Fernandes-Henriques1,2, Yuval Guetta3, Mia Sclar2
1Biology Program, The Graduate Center, CUNY, 10016.
概括
截至主体无名 - 腹 (SI / VP) 的内膜 (IL) 皮层途径限制了在灭绝学习期间的恐惧反应. 这一途径与IL→底侧桃体 (BLA) 途径不同,对于调节防御行为至关重要.
科学领域:
- 神经科学是一个神经科学.
- 行为神经科学 行为神经科学
- 学习和记忆的学习和记忆
背景情况:
- 恐惧灭绝对于适应非威胁性刺激至关重要.
- 众所周知,下边膜 (IL) 皮层和底侧杏仁体 (BLA) 中介于灭绝记忆检索.
- 参与灭绝学习的IL途径仍然不太了解.
研究的目的:
- 研究IL→substantia innominata-ventral pallidum (SI/VP) 途径在恐惧灭绝学习中的作用.
- 为了比较IL→SI/VP路径与已知的IL→BLA路径在灭绝过程中的功能.
- 阐明在学习和检索过程中活跃的特定IL子路径.
主要方法:
- 在雄性小鼠中,逆行追踪将IL投影映射到SI/VP和BLA.
- 结合跟踪与cFos表达来评估学习和检索期间的路径活动.
- 在IL神经元的体外电生理学记录.
- 在IL→SI/VP路径的光遗传失活化.
主要成果:
- 对SI/VP的IL投影来自表面 (L2/3) 和深层 (L5),比IL→BLA投影更密集.
- 在灭绝学习过程中,L5 IL→SI/VP 途径的活性增加;在恢复过程中,L2/3 IL→BLA 途径的活性增加.
- 与检索相比,IL→SI/VP神经元在灭绝学习过程中表现出更高的刺激性.
- 不激活IL→SI/VP通路会在灭绝和重新灭绝期间增强防御结.
结论:
- IL→SI/VP路径积极参与恐惧灭绝学习,其功能是限制防御性结.
- IL→BLA路径更多地参与了灭绝记忆检索.
- 在学习过程中,IL充当了一个动态开关板,调节与SI/VP的通信,并在检索过程中调节与BLA的通信.
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