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使用在半孔石架上固定的双酶系统增强亚染料降解

Shun-Ichi Matsuura¹, Takeshi Ikeda², Manami Chiba¹

¹Research Institute for Chemical Process Technology, National Institute of Advanced Industrial Science and Technology (AIST), 4-2-1 Nigatake, Miyagino-ku, Sendai, Miyagi 983-8551, Japan.

Journal of bioscience and bioengineering

|April 22, 2025

概括

在半孔性二氧化上酶固定增强甲基红色染料的降解. 将酶与结标签融合,可以提高活性和耐久性,克服聚合问题,以有效地去除染料.

科学领域:

生物催化和酶工程生物催化和酶工程
材料科学材料科学材料科学
环境生物技术环境生物技术

背景情况:

在溶液中,自由的亚缩酶 (AzoR) 和葡萄糖脱酶 (GDH) 聚合并失活.
酶固定对于生物催化过程中的稳定性和可重复使用性至关重要.
半孔为酶固定提供了一个有前途的支架,因为它的高表面积和有序的孔隙.

研究的目的:

开发一种稳定高效的双酶系统,用于色剂降解.
通过固定化来克服酶聚合和失活的问题.
为了增强甲基红色降解使用Si标记酶固定在半孔上.

主要方法:

亚索减少酶 (AzoR) 和葡萄糖脱酶 (GDH) 与结合蛋白 (Si-tag) 的融合.
将Si标记酶固定在半孔和无孔支上.
评估甲基红色降解效率,酶活性和可重复使用性.

主要成果:

与无孔相比,对中孔的固定显著增加了甲基红色降解比率.
Si-tag融合增强了固定AzoR和GDH的活性和耐久性,防止了GDH的脱吸.
双酶-半孔二氧化复合材料在甲基红色降解中表现出优越的耐用性和重复性.

结论:

通过Si-tag在半孔上进行介导的固定,有效地克服了酶聚合和失活.
开发的双酶系统为色剂降解提供了高效和稳定的生物催化剂.
这种方法为废水处理和染料去除的工业应用提供了有前途的战略.

关键词:

亚索染料是一种染料.亚还原酶可以减少亚.同酶的再生共酶的再生葡萄糖脱水酶的使用固定化的酶.半孔性二氧化材料是一种半孔性二氧化材料.结合蛋白是一种结合的蛋白质.

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