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来自贝赫特尿道炎患者中性粒细胞的蛋白质组概况及其性别差异

Rong Liu¹, Qingfeng Wang¹, Qingyan Jiang¹

¹Ophthalmology Medical Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory for the Prevention and Treatment of Major Blinding Eye Diseases, Chongqing Branch (Municipality Division) of National Clinical Research Centre for Ocular Diseases, Chongqing, People's Republic of China.

|April 22, 2025

概括

在贝赫特卵膜炎 (BU) 中的中性粒细胞蛋白质变化揭示了活性中性粒细胞外细胞陷形成和抑制的亡. 男性患者表现出增强的炎症和抑制的亡,有助于疾病发病和性别差异.

科学领域:

眼科医生眼科眼科
免疫学免疫学免疫学
蛋白质组学是指蛋白质组学

背景情况:

贝赫特膜炎 (Behcet's uveitis,BU) 是一种严重的视力威胁性疾病,男性占主导地位.
中性粒细胞功能障碍与BU的发病有关,但详细的蛋白质组变化仍然不清楚.

研究的目的:

在活跃的BU患者中研究中性粒细胞的蛋白质组概况.
阐明受影响的功能途径,并确定中性粒细胞变化的基于性别的差异.

主要方法:

从活跃的BU患者和健康对照组 (HCs) 获得的外围中性粒细胞的蛋白质组分析.
对差异表达蛋白 (DEP) 的功能丰富分析.
蛋白质组数据与单细胞RNA测序 (scRNA-seq) 数据的综合分析.

主要成果:

与HC相比,在BU中性粒细胞中确定了82个上调和516个下调的DEP.
发现活化的中性粒细胞细胞外陷 (NET) 形成途径和抑制的核酸代谢和亡.
与女性相比,男性BU患者表现出增强的中性粒细胞介导炎症途径和抑制的亡.
综合性分析将性别差异与特定中性粒细胞子集的炎症增强和亡/核酸代谢受损联系在一起.

结论:

中性粒细胞的蛋白质变化和功能变化,包括 NET 形成和亡,在 BU 病变发生过程中至关重要.
性别特定的蛋白质组特征有助于男性在BU发病率和严重程度方面的偏见.
这些发现提供了BU病理生理学和潜在的治疗点的见解.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.贝赫特的脑膜炎贝赫特的脑膜炎中性粒细胞过活化核酸代谢的核酸代谢蛋白质组学是指蛋白质组学

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