新的精神活性物质埃斯凯特胺引起内分泌干扰效应和发育毒性
Si-Ying Li1,2, Dongdong Ma1,2, Wen-Jun Shi1,2
1SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China.
在水中的石胺 (ESK) 损害了斑马鱼的发育,并破坏了它们的内分泌系统,显示了双相剂量反应. 这凸显了ESK对鱼群的生态风险.
科学领域:
- 环境毒理学环境毒理学
- 水生生态毒理学 水生生态毒理学
- 内分泌学 在内分泌学.
背景情况:
- 精神活性物质埃斯凯特胺 (ESK) 在全球地表水中越来越多地被检测到.
- 它对水生生物,特别是鱼类的潜在毒性和内分泌干扰作用尚不清楚.
研究的目的:
- 调查斑马鱼中乙胺 (ESK) 的发育毒性和内分泌干扰潜力.
- 评估环境相关的ESK度对斑马鱼胚胎和细胞的影响.
主要方法:
- 斑马鱼胚胎和ZF4细胞在受精后的24小时和14天内暴露在不同度的ESK (0,0.12,1.02,10.6微克L-1) 中.
- 分析了基因表达,细胞周期,黄囊参数和维特洛根因 (VTG) 水平.
- 用KEGG路径分析和分子对接来理解分子机制.
主要成果:
- ESK对斑马鱼胚胎发育表现出双相剂量依赖的影响,低/中度促进生长,高度抑制生长.
- 暴露于ESK上调了*vtg*的表达,并破坏了细胞循环.
- 凯格分析显示了对昼夜节律,核酸切除修复和雌激素信号通路的显著影响.
- 升高的VTG水平和分子对接证实了与雌激素受体的相互作用,表明内分泌干扰.
结论:
- 乙胺 (ESK) 在斑马鱼中显示出显著的发育毒性和内分泌干扰作用.
- 这些发现强调了水生环境中ESK污染带来的生态风险,并需要进一步调查其对鱼类健康的影响.
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