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Updated: May 10, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

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在Streptococcus pneumoniae中进行基因组工程的Make-or-break主要编辑

Monica Rengifo-Gonzalez1, Maria-Vittoria Mazzuoli1, Axel B Janssen1

  • 1Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Biophore Building, CH-, Lausanne, Switzerland.

Nature communications
|April 22, 2025
PubMed
概括

我们开发了Make-or-break Prime Editing (mbPE),这是一种用于精确细菌基因组工程的新型CRISPR-Cas9工具. 这种方法简化了Streptococcus pneumoniae的遗传修饰,增强了未来的研究和应用.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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