使用区域选择性Baeyer-Villiger单氧化酶生物催化生产3基酸前体
Melissa De Angelis1, Danilo Correddu1, Fabio Bucciol2
1Department of Life Sciences and Systems Biology, University of Torino, Via Accademia Albertina 13, Torino, 10123, Italy.
Scientific reports
|April 22, 2025
概括
这项研究展示了一种可持续的化学酶法,使用Acinetobacter辐射抵抗剂Baeyer-Villiger单氧化酶 (Ar-BVMO) 来将纤维素衍生的丁酸转化为有价值的3-乙基酸 (3-APs),这是3-基酸 (3-HP) 的前体.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 绿色化学和可持续的过程
- 生物技术和可再生化学品
背景情况:
- 贝耶-维利格单氧化酶 (BVMOs) 是一种酶,在温和的条件下催化氧化成 Ester.
- BVMOs可以将纤维素衍生的基氨酸转化为3-基烯酸 (3-AP),这是3-基酸 (3-HP) 的前体,这是一个关键的平台化学物质.
- 从可再生资源中开发高效和可持续的3HP生产路线对化学工业至关重要.
研究的目的:
- 评估Acinetobacter辐射抵抗BVMO (Ar-BVMO) 作为生物催化剂,用于将基酸盐转化为3-APs.
- 优化生物转化过程以提高产量和效率.
- 从可再生纤维素原料中开发一种可持续的化学酶方法来生产3-HP前体.
主要方法:
- 选Ar-BVMO活性与甲基,乙基和乙基氨酸基底.
- 使用大肠杆菌全细胞过度表达Ar-BVMO以增加产品产量.
- 微波辅助的纸醇化,从纤维素中产生丁乙烯酸.
- 在细胞溶解物中使用Ar-BVMO对丁酸的生物转化.
主要成果:
- Ar-BVMO 显示了更高的催化效率 (4 倍) 和区域选择性 (4: 1) 对丁乙烯酸转化.
- 全细胞生物转化在9个小时内实现了百分之八十五的丁酸转化.
- 微波辅助醇化产生布利酸,从纸中具有92.7%的选择性.
- 在细胞溶解物中的Ar-BVMO在24小时内完全转化为丁酸,在不经过净化的情况下产生3-HP前体.
结论:
- Ar-BVMO是一种有效的生物催化剂,用于将丁酸乙烯转化为3-AP,使用丁酸乙烯的最佳性能.
- 化学酶方法,结合微波辅助合成和Ar-BVMO生物转化,提供了从纤维素到3-HP前体的可持续途径.
- 这项研究强调了利用可再生原料和生物催化剂生产有价值的平台化学品的潜力.
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