血液采集技术和处理影响细胞核,并补充血小板产品的内容
Yasmin E S de Wit1, Ido J Bontekoe2, Rick van Andel3
1Department of Immunopathology, Sanquin Blood Supply, Amsterdam, the Netherlands.
Vox sanguinis
|April 22, 2025
概括
血小板缩物 (PCs) 显示出更好的激活,而不是布菲外套PCs. 布菲外套PC具有更高的核细胞和中性粒细胞激活标志物,表明潜在的不良反应.
科学领域:
- 输血医学 输血医学
- 免疫学 免疫学 免疫学
- 血液学 血液学 血液学
背景情况:
- 用于输血的血小板缩剂 (PC) 是通过全血献血 (buffy-coat,BC-PC) 或异血 (apheresis-PC) 制备的.
- 制备方法可能会影响损伤相关分子模式 (DAMP) 和补充激活,可能会影响输血不良反应 (AR).
研究的目的:
- 为了比较血小板激活,DAMPs,和补充激活之间的白血病减小的光-PC和布菲外套PC (BC-PC).
主要方法:
- 在血或血小板添加剂溶液 (PAS-E) 中储存的阿菲雷西斯-PC和BC-PC (从5名捐赠者汇总) 的比较.
- 直接在生产后测量CD62P+血小板,核细胞,线粒体DNA (mtDNA),血和补充激活产品.
- 基于捐赠时间 (上午与下午) 的BC-PCs分组.
主要成果:
- 与BC-PC相比,Aphaeresis-PC在刺激后表现出较高的CD62P+血小板水平.
- 显著增加的核细胞和C4b/c的水平在BC-PC和阿菲雷斯-PC.发现.
- 早晨BC-PC中弹性酶-α1-抗素复合体 (EA) 的较高水平表明中性粒细胞活化增加.
结论:
- 与BC-PC相比,Aphaeresis-PC显示出更高的激活潜力.
- BC-PC含有高核细胞和EA,可能是由于残留的中性粒细胞.
- BC-PC显示补充激活产品的增加,可能与制备方法有关.
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