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使用基于昆虫的菜食品废弃物的新方法,用于将基因向癌症组织

Shadi Majd-Marani¹, Ali Eftekhari², Sabry G Elias³

¹Department of Plant Production and Genetics, University of Mohaghegh Ardabili, Ardabil, Iran.

Scientific reports

|April 22, 2025

概括

研究人员从粉虫废物和菜提取物中开发了新的氧化铁纳米颗粒. 这些生物相容的纳米颗粒有效地将基因治疗剂运送到乳腺癌细胞和瘤中,使用磁性向.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
癌症治疗癌症治疗

背景情况:

开发有针对性的药物输送系统对于有效的癌症治疗至关重要.
氧化铁纳米粒子为磁性准和成像提供了潜力.
纳米颗粒的可持续合成方法越来越重要.

研究的目的:

通过可持续的食品废弃物来源合成和表征新型Fe3O4纳米颗粒.
通过聚合物使这些纳米粒子具有功能,用于siRNA/DNA加载和有针对性的传递.
评估功能化纳米粒子的生物相容性,基因传递效率和磁性准能力.

主要方法:

从 (Tenebrio molitor) 幼虫合成的Fe3O4纳米颗粒被食了菜 (Spinacia oleracea) 提取物.
用高分支精氨酸-聚乙烯糖醇-叶酸 (FHSPF) 和精氨酸-聚乙烯糖醇-叶酸 (FSMPF) 聚合物涂覆的纳米粒子,装载有siRNA或DNA.
使用DLS,H-NMR,FTIR,EDX,Zeta潜力和TEM进行表征.
评估生物相容性 (细胞毒性测试),DNA释放,基因转移效率 (MCF-7细胞,光显微镜,流动细胞计),以及使用外部磁场在小鼠体内瘤向.

主要成果:

涂层纳米粒子增加了泽塔潜力,DNA释放,封装和基因传递效率.
这些纳米粒子具有较低的细胞毒性和良好的生物相容性.
增加siRNA或DNA度提高了基因传输效率.
在体内,FHSPF2纳米颗粒显示出高生物相容性,并有效地将siRNA-FAM传递给乳腺癌细胞和瘤.
外部磁场显著增强了瘤部位的纳米粒子吸附.

结论:

使用食品废物可实现Fe3O4纳米颗粒的可持续合成.
功能化纳米颗粒显示出作为癌症治疗的生物相容性和高效基因传递载体的承诺.
磁性准显著改善了瘤部位的纳米粒子积累,增强了治疗潜力.

关键词:

乳腺癌是什么? 乳腺癌是什么?基因准是基因的目标.磁性纳米粒子是一种磁性纳米粒子.食虫是一种食虫.这就是siRNA-FAM.

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