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双差积累SERS战略用于快速分离和检测益生菌Bacillus内和植物细胞

Shijie Liu¹, Qian Ding¹, Yueyu Bai²

¹College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China; Sauce braised and prefabricated products modern production school enterprise research and development center, Henan Agricultural University, Zhengzhou 450002, PR China.

Food research international (Ottawa, Ont.)

|April 23, 2025

概括

一个新的SERS策略快速分离并检测细菌内和植物细胞. 这种方法在食品样本中实现了高精度,有助于益生菌生产安全.

科学领域:

分析化学分析化学
生物技术是生物技术.
食品科学食品科学食品科学

背景情况:

益生菌细菌有一个重要的全球市场.
目前的方法缺乏快速分离和检测Bacillus内和植物细胞.
准确的区分对于益生菌生产的质量控制至关重要.

研究的目的:

开发一种快速而灵敏的方法来区分Bacillus内和植物细胞.
为了在复杂的食品矩阵中实现高效的分离和检测.
加强对益生菌Bacillus在工业化生产中的安全监测.

主要方法:

开发一种"双差积累"的表面增强拉曼光谱 (SERS) 策略.
使用Fe3O4@AgNPs@Van和AuNPs-PA-COFs基板进行分离和丰富.
应用主要组件分析 (PCA) 和部分最小平方差分分析 (PLS-DA) 进行分类.

主要成果:

实现了细菌内和植物细胞的快速分离和丰富.
产生了"悬崖般"的10倍SERS信号差异,以实现清晰的差异化.
在食品样本中,已证明检测极限低于10 CFU/mL,准确度高 (95.45%98.52%).

结论:

"双差分积累"SERS策略为区分细菌细胞类型提供了一个强大的方法.
这种技术支持高密度培养的维护和工业益生菌生产的安全监测.
该方法为全球益生菌市场的质量控制提供了重大进步.

关键词:

末子是一种内体.益生菌细菌是一种益生菌.快速检测快速检测快速检测快速检测这就是 SERS SERS.分离和净化工作.植物细胞是植物细胞.

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