相关实验视频
Updated: Jun 23, 2026

06:51
Film Extrusion of Crambe abyssinica/Wheat Gluten Blends
Published on: January 17, 2017
9.9K
通过使用转谷氨酸酶的高湿度挤出制备的大豆-大麻-小麦蛋白质肉类类似物的物理化学调制
Zhouliang Sun1, Wanhui Zhang1, Fengkai Zhang1
1College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Food research international (Ottawa, Ont.)
|April 23, 2025
概括
转胺酶 (TGase) 通过增强蛋白质交叉链接,显著改善肉类模拟的质地和保持水的容量. 这种酶促进大豆,大麻和小麦质等植物性产品中优质纤维的形成.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 材料科学 是一种材料科学.
背景情况:
- 转谷氨酸酶 (TGase) 对于高湿度挤出肉类类似物 (HMEMA) 中的蛋白质交叉连接至关重要.
- 对于TGase对整个挤出区的蛋白质结构和质量的特定影响尚不完全理解.
- 从各种植物蛋白质中研究TGase在HMEMA中的作用对于产品开发至关重要.
研究的目的:
- 研究TGase对HMEMA的物理化学特性和质感的影响.
- 分析TGase对蛋白质结构和不同挤出区内的分子间相互作用的影响.
- 阐明TGase影响植物性肉类类似物纤维形成的机制.
主要方法:
- 这是一个很好的选择.
- 死亡停止,死亡停止.
- 实验用于分析使用和不使用TGase产生的HMEMA.
- 进行了微观结构分析,蛋白质结构 (二次结构) 的确定和分子间力量的评估.
- 使用大豆蛋白 (SP),大麻蛋白 (HP) 和小麦质 (WG) 制备的HMEMA.
主要成果:
- 加TGas (0.18%) 增加了15.4%的纹理化和42.5%的水容量 (WHC).
- 经过TGAS处理的HMEMA表现出明显的纤维结构,与对照者的分层形态不同.
- TGase增强了结和疏水相互作用,改变了蛋白质的二次结构 (在化区减少α-螺旋/β-片,在挤出区增加).
结论:
- TGase在增强来自各种植物蛋白质的HMEMA的纹理特性和质量方面发挥着关键作用.
- 该酶在挤出过程中促进蛋白质扩张和重组,从而改善纤维形成.
- 这些发现为优化MA生产使用各种植物性蛋白质来源提供了宝贵的见解.
相关概念视频
Mechanical Protein Functions
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
Mechanical Protein Function
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
Production of Pharmaceuticals
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...

