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葡萄糖和谷氨酸驱动的de novo核酸合成促进了WSSV在中的复制

Cong-Yan Chen¹, Chih-Ling Chen², Yen Siong Ng²

¹Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan.

Cell communication and signaling : CCS

|April 23, 2025

概括

白斑综合征病毒 (WSSV) 劫持了的新陈代谢,重新编程葡萄糖和谷氨酸通路,以推动病毒复制所必需的 de novo 核酸合成. 这种代谢重编程为控制水产养殖中的WSSV提供了潜在的目标.

科学领域:

病毒学病毒学
代谢学代谢学代谢学
水产养殖病理学水产养殖病理学

背景情况:

病毒,包括白斑综合征病毒 (WSSV),依赖宿主细胞代谢进行复制.
在中感染WSSV会诱导有氧糖解和谷氨醇解,这对于病毒需求至关重要.
在WSSV感染期间激活了de novo核酸合成,但其与宿主代谢的联系尚不清楚.

研究的目的:

调查葡萄糖和谷氨酸代谢在WSSV复制过程中的de novo核酸合成中的作用.
为了阐明WSSV如何重新编程宿主代谢途径来产生病原体.

主要方法:

使用同位素标记的葡萄糖和谷氨胺 ([U-¹³C]葡萄糖,[A-¹⁵N]谷氨胺) 作为 (Litopenaeus vannamei) 的标记剂.
测量了与核酸代谢相关的基因表达和酶活性.
使用dsRNA介导的基因沉默和药理抑制剂 (Ras-PI3K-Akt-mTOR通路) 来评估调控机制.

主要成果:

在WSSV复制 (12hpi) 期间确认了新核酸合成的显著激活.
已证明葡萄糖代谢支持氨酸合成,而谷氨酸则对氨酸和氨酸的合成作出贡献.
通过基因沉默验证了de novo核酸合成在WSSV复制中的关键作用;Ras-PI3K-Akt-mTOR通路的抑制对病毒复制没有一致的影响.

结论:

感染WSSV引发了葡萄糖和谷氨酸的特定代谢重编程,以增强的新核酸合成.
这些代谢变化为WSSV复制和致病的重要前体.
在养殖中确定了WSSV控制策略的潜在目标.

关键词:

酸酸盐路径中的酸.华堡效应是什么意思? 华堡效应白是什么意思白是什么意思白是什么意思白色斑点综合征病毒病毒.在 de novo 核酸代谢中.在体内稳定同位素追踪的代谢组分.

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