通过转录因子LlWOX11在Lilium lancifolium中识别的cis作用元素的识别
Jingyi Bai1,2, Panpan Yang2, Mengmeng Bi2
1College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming, Yunnan, China.
Physiologia plantarum
|April 23, 2025
概括
兰花LlWOX11结合了新的DNA动机,揭示了它在调节基因表达和促进植物球泡形成方面的作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物遗传学 植物遗传学
- 发育生物学是发展生物学.
背景情况:
- WOX转录因子通过与特定的DNA元素结合来调节植物的发育.
- 来自Lilium lancifolium的LlWOX11已知可以促进球泡的形成,并与TTAATGAG图案结合.
研究的目的:
- 为了识别由LlWOX11.11识别的新型DNA图案.
- 阐明LlWOX11介导的球泡形成背后的分子机制.
主要方法:
- 转录因子为中心的酵母1杂交 (TF为中心的Y1H) 试验用于识别DNA结合动机.
- 酵母一混合 (Y1H) 测试以确定核心动机序列.
- 双化酶试验 (LUC),电泳运动转移试验 (EMSA) 和染色体免疫沉 (ChIP) 以验证在植物中的结合.
- 对LllWOX11对LllRR9转录的影响的分析.
主要成果:
- LlWOX11识别了五种不同的七基DNA基因,包括三种已知的cis作用元素和两种新型基因 (TGCGAAA,TCCATCA).
- 在植物验证中证实LlWOX11与这些动机结合.
- 发现LllWOX11通过结合这些促进因子来抑制LllRR9的转录.
- 这种抑制最终促进了Lilium lancifolium中的球泡形成.
结论:
- LlWOX11与以前所知的更广泛的DNA动机结合.
- LlWOX11通过抑制 LlRR9 转录来调节球泡的形成.
- 这些发现提高了对WOX蛋白功能和植物发育机制的理解.
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