从谷物中分离出来的Fusarium Proliferatum菌株的产生毒素的能力
O P Gavrilova1, A S Orina1, T Yu Gagkaeva1
1All-Russian Institute of Plant Protection, St. Petersburg, 196608 Russian Federation.
Acta naturae
|April 23, 2025
概括
这项研究从谷物中确定了12个Fusarium proliferatum菌株,证实了它们的分类学和异质性交配. 所有菌株都产生了显著水平的烟素,贝奥维里辛和莫尼利福尔菌毒素.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物病理学 植物病理学
- 食品安全 食品安全
背景情况:
- Fusarium proliferatum是一种广泛传播的真菌,感染了各种植物.
- 这种真菌产生了一系列的真菌毒素,含量各不相同.
- 谷物中的菌毒素污染对食品安全构成重大风险.
研究的目的:
- 从分类学上识别Fusarium proliferatum从小麦,麦和玉米粒中分离出来的菌株.
- 为了确定已识别的菌株的交配类型.
- 量化这些菌株的二次毒性代谢物产量.
主要方法:
- 对翻译延长因子EF-1α,β-tubulin和RNA聚合酶II基因片段进行家族遗传学分析,用于菌株识别.
- 为确定交配类型而进行的基因特异性PCR.
- 高性能液体染色学-并联质谱学 (HPLC-MS/MS) 用于毒素量化.
主要成果:
- 通过遗传学分析证实了12个F. proliferatum菌株,形成了一个独特的分类.
- 所有菌株都表现出异体性交配, MAT1-1异形的频率更高.
- 在所有菌株中检测到显著水平的烟素B1,B2,B3,波韦里辛和莫尼利福尔明.
结论:
- 这项研究证实了F. proliferatum从谷物谷物中获得的菌株的同一性和交配特征.
- 所有分析的菌株都能够产生有害的真菌毒素,包括烟素和贝奥维里辛.
- 这项研究强调了在主食作物中与F. proliferatum相关的潜在真菌毒素风险.
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