转录因子VvbHLH137 积极调节葡萄 (葡萄藤) 中的安托素积累 (Vitis vinifera)
Zaozhu Niu1, Zhichao Zhang1, Yanzhuo Zhao1
1Shijiazhuang Institute of Fruit Trees, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050061, China.
Plants (Basel, Switzerland)
|April 23, 2025
概括
研究人员确定VvbHLH137是葡萄中安托氨酸合成的关键调节剂. 这种转录因子的过度表达增强了果的颜色,有助于分子繁殖以增强葡萄的色彩.
科学领域:
- 植物分子生物学 植物分子生物学
- 农业科学 农业科学
- 生物化学 生物化学
背景情况:
- 葡萄的颜色对于水果的质量至关重要,这是由素积累驱动的.
- 基本螺旋环-螺旋环 (bHLH) 蛋白质是氨酸合成的关键调节者,但许多葡萄bHLHs没有特征.
- 了解这些调节者对于改善葡萄品种和水果质量至关重要.
研究的目的:
- 识别和描述参与葡萄中安托素生物合成和果色调的新型bHLH转录因子.
- 阐明"摩尔多瓦"葡萄品种中安托素积累的监管机制.
- 提供分子洞察力,以提高葡萄的颜色.
主要方法:
- 超高性能液态色谱-并联质谱 (UPLC-MS/MS) 用于果成分分析.
- 转录基因组测序以识别安托素通路中的基因和转录因子.
- 基因表达分析,包括Arabidopsis thaliana*中的过度表达,以及蛋白质与蛋白质相互作用研究.
主要成果:
- 马尔维丁衍生物被确定为"摩尔多瓦"葡萄中主要的安托西亚宁.
- 鉴定出了40个基因和几个与安东合成相关的转录因子 (TF),其中VvCHS2表达最高.
- 鉴定出VvbHLH137是安托氨酸合成的积极调节剂,在过度表达时增强含量.
- VvbHLH137与VvMYB蛋白相互作用,并直接刺激VvDFR和VvF3H转录.
结论:
- 在调节葡萄色彩方面,VvbHLH137通过促进氨酸合成,发挥着重要作用.
- 这些发现提供了对葡萄中安托素生物合成途径的宝贵分子洞察力.
- 这项研究支持对葡萄品种的分子育种策略的开发,这些葡萄品种具有改善的色彩.
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