相关实验视频

Updated: May 5, 2026

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

Published on: April 16, 2019

牛乳杆菌 (Lactobacillus delbrueckii) 的一个亚种作为老年患者中新兴的人类泌尿病原体的allosunkii和lactis

François Guérin^1,2,3, Mohamed Sassi¹, Francois Gravey^4,5

¹INSERM UMR 1230 BRM, Université de Rennes, Rennes, France.

Journal of clinical microbiology

|April 23, 2025

概括

乳酸菌delbrueckii,特别是allosunkii亚种,是老年妇女尿道感染 (UTI) 的重要原因. 这项研究强调需要重新评估实验室实践,并改善对这些被诊断不足的感染患者的护理.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
基因组学就是基因组学.

背景情况:

乳杆菌delbrueckii很少与人类尿路感染 (UTI) 有关.
关于其临床意义,抗微生物敏感性和尿路分离物中的基因组特征的数据有限.
这项研究解决了关于L.delbrueckii作为尿路感染病原体的知识差距.

研究的目的:

分析L.delbrueckii尿道分离物的临床,微生物学和基因组数据.
确定L.delbrueckii在尿路感染中的临床意义和抗菌敏感性.
描述临床L.delbrueckii分离的基因组多样性.

主要方法:

从住院患者 (2014-2016) 中分离的L.delbrueckii的回顾性分析.
使用MALDI-TOF质谱法进行物种识别.
通过微稀释进行抗微生物敏感性测试.
全基因组测序 (WGS) 和比较基因组分析 (MLST,ANI,核心基因组SNP).

主要成果:

从老年患者 (平均年龄84岁,96%为女性) 的尿液中检测到48个L.delbrueckii分离物.
44%的患者被诊断出患有尿路感染;分离体显示显著的细菌性尿 (≥10^5 CFU/mL).
大多数分离体对β-乳酸盐敏感,对氏素和甲尼醇耐药;两种对四环素耐药的分离体含有tet(W) 基因.
基因组分析显示,L.delbrueckii亚种allosunkii (83%) 和乳类 (17%),占主导地位.

结论:

德尔布鲁埃基,特别是allosunkii和lactis亚种,起着重要的尿病原作用,特别是在老年女性患者中.
临床实验室应重新考虑从尿液中排放乳酸菌作为污染物的常规排放.
建议选择适当的抗生素,如β-乳糖类药物,用于治疗这些未被确诊的尿路感染.

关键词:

牛乳杆菌 (Lactobacillus delbrueckii) 是一种植物.这是一个UTI,UTI是UTI.乳酸细菌菌 (lactobacilli) 是一种乳酸菌.亚种亚种亚种亚种尿液尿液尿液

更多相关视频

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

Published on: June 16, 2022

Author Spotlight: Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Author Spotlight: Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Published on: June 23, 2023

相关概念视频

Urinary Tract Infection II: Pathophysiology

Urinary Tract Infection II: Pathophysiology

The pathophysiology of urinary tract infections (UTIs) encompasses several progressive stages, beginning with bacterial colonization and culminating in potential systemic complications if untreated. UTIs are primarily initiated by bacteria, such as Escherichia coli, which often originate from the gastrointestinal tract and migrate to the urinary system through the periurethral area. This migration can occur via several routes, including improper hygiene practices, sexual activity, or...

Urine Studies II: Urine Culture and Sensitivity Test

Urine Studies II: Urine Culture and Sensitivity Test

A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...

Bacterial Phylum Firmicutes

Bacterial Phylum Firmicutes

Firmicutes is a diverse phylum of Gram-positive bacteria characterized by a low GC content in their genomes. This phylum includes organisms with monoderm or diderm cell envelopes, highlighting a complex evolutionary history. Firmicutes comprises several major orders, including Lactobacillales, Clostridiales, and Bacillales, which exhibit remarkable diversity in their morphology, metabolism, and ecological roles.The order Lactobacillales includes lactic acid bacteria, which are fermentative...

Development of Human Microbiota

Development of Human Microbiota

The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from...

Microbiota of the Urogenital Tract

Microbiota of the Urogenital Tract

The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...

Clinical Significance of Antibiotic Resistance

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...