PABPN1将母体转录的多化和翻译结合到小鼠卵细胞质成熟过程中
Xing-Xing Dai1, Yu-Ke Wu2, Shuai-Bo Pi2
1Department of Obstetrics and Gynecology, Center for Reproductive Medicine, the Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, 322000, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|April 23, 2025
概括
聚氨基酸结合蛋白核1 (PABPN1) 对于小鼠卵细胞成熟至关重要,调节母性mRNA的聚氨基化和降解. 它的缺失阻止了生殖囊泡的分解,并导致介质性错误.
科学领域:
- 生殖生物学 生殖生物学
- 分子细胞生物学 分子细胞生物学
- 发展生物学 发展生物学
背景情况:
- 母亲的mRNA多化对卵细胞成熟至关重要.
- 聚乙烯酸结合蛋白核1 (PABPN1) 调节体细胞和生长卵细胞中的mRNA.
研究的目的:
- 为了研究PABPN1在成熟的小鼠卵细胞中介性成熟中的作用.
- 阐明PABPN1控制介质进展的分子机制.
主要方法:
- 使用Zp3-Cre.Cre.在生长中的小鼠卵细胞中选择性淘汰Pabpn1.
- 包含多A的全长RNA异形序列 (PAIso-seq) 和转录组序列.
- 进行救援实验以验证PABPN1功能.
主要成果:
- 在Pabpn1中,淘汰阻止了生殖囊泡的分解,损害了CDK1的激活,并导致了螺旋/染色体异常.
- PABPN1协调母体mRNA的多基解和降解,这对成熟至关重要.
- 关键的PABPN1域与多聚酶相互作用,并招募目标mRNA.
结论:
- 细胞质PABPN1在哺乳动物卵细胞成熟方面具有生理意义.
- PABPN1整合了母体转录的多基化,翻译和降解,用于介质性进展.
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