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针对SARS-CoV-2的双特异性和三特异性抗体的设计和表征

Jiayang Wang¹, Qi Qian², Yushan Jiang¹

¹BSL-3 Laboratory (Guangdong), Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China.

|April 23, 2025

概括

新的多种类型抗体显示出治疗COVID-19的前景. 这些新型双特异性和三特异性抗体有效中和SARS-CoV-2变体,并证明了体内治疗效果,为新兴传染病提供了潜在的新疗法.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
生物技术是生物技术.

背景情况:

由SARS-CoV-2引起的COVID-19大流行需要新的治疗方法,因为新出现的免疫逃避变种.
现有的治疗方法,如单克隆抗体,面临着奥米克朗BA.1,BA.2,XBB.1和EG.5.5等变异的挑战.
迫切需要广泛的抗病毒药物来对抗SARS-CoV-2不断变化的威胁.

研究的目的:

开发用于COVID-19治疗的新型多种类型抗体.
评估针对SARS-CoV-2变种的双特异性和三特异性抗体的疗效.
探索抗体工程在应对新兴传染病方面的潜力.

主要方法:

重组双特异性 (IgG-scFv) 和三特异性 (Fab片段) 抗体在HEK 293细胞中表达.
在试验室中使用伪病毒中和试验评估了疗效.
在体内治疗潜力通过BALB/c小鼠的病毒挑战研究来评估.

主要成果:

双特异性抗体显示出显著的伪病毒中和,IC50值从8到591 ng/mL.
三种特异性抗体表现出优异的中和,IC50值在5至27ng/mL之间.
双特异性和三特异性抗体都在SARS-CoV-2感染的小鼠模型中显示出治疗疗效.

结论:

开发的多种类型抗体为COVID-19提供了潜在的新疗法选择.
多种类型的抗体策略在对抗新兴病毒病原体方面具有重大前景.
这项研究有助于开发针对传染病的基于抗体的先进对策.

关键词:

在 COVID-19 疫情中,这就是SARS-CoV-2病毒.两种特异性的抗体.三种特异性的抗体.

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