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巨宿主 Porphyromonas gingivalis 是白内障发展的危险因素

Dongzhe Zhang¹, Junwei Qu¹, Cuncun Ke¹

¹Division of Vision Science, Joint National Laboratory for Antibody Drug Engineering, Henan University, Kaifeng, China.

Investigative ophthalmology & visual science

|April 23, 2025

概括

Porphyromonas gingivalis (PG) 感染与白内障的发展有关,特别是在糖尿病和高近视白内障等特定类型中. PG通过巨细胞进入透镜表皮,通过氧化应激和炎症促进白内障的进展.

科学领域:

眼科医生眼科眼科
微生物学微生物学
免疫学免疫学免疫学

背景情况:

在全球范围内,白内障是导致视力丧失的主要原因.
细菌感染在白内障发生中的作用仍然是积极研究的领域.

研究的目的:

调查Porphyromonas gingivalis (PG) 感染与白内障的发展之间的联系.
阐明PG可能导致白内障形成的机制.

主要方法:

使用PCR和FISH检测PG 16s核糖体RNA基因在水和前囊组织中.
针对RpgA表达和纤维化标志物的免疫光.
反应性氧物种和亡的流动细胞计.
用于差异基因表达分析的RNA深度测序.

主要成果:

在43.3%的白内障水性幽默样本中检测到PG DNA,明显高于绿内障样本.
在白内障前组织的82-94%中发现了PG.
在透镜表皮中确定了PG阳性巨细胞,与大鼠模型中纤维化标志物增加和氧化应激相关.
RNA测序揭示了与PG感染白内障组织上皮质-介质细胞过渡,氧化应激和细胞死亡相关的途径的上调.

结论:

慢性 Porphyromonas gingivalis 感染可以通过巨细胞到达透镜表皮,从而导致白内障的发展.
PG感染通过涉及氧化应激,亡和上皮-介质酶过渡的机制促进白内障发生.
Porphyromonas gingivalis 是一种新的白内障发展的危险因素.

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