在牛中对非结核菌菌群和Mycobacterium abscessus进行高通量测序
Siqi Chen1,2, Mengda Liu2, Yan Li3
1College of Animal Science and Technology, Shihezi University, Shihezi 832003, China.
Veterinary sciences
|April 23, 2025
概括
包括Mycobacterium abscessus (Mab) 在内的非结核菌 (NTM) 在牛场和屠宰场环境中被检测到. 这项研究突出了这些机会性病原体的流行情况,并评估了它们的动物感染潜力,以改善控制策略.
科学领域:
- 环境微生物学环境微生物学
- 兽医公共卫生 兽医公共卫生
- 细菌基因组学是一种细菌基因组学.
背景情况:
- 非结核菌 (NTM) 是机会性病原体,对人类和动物健康有重大影响.
- 菌根 (Mab),一个快速生长的NTM,表现出抗菌素耐药性,并导致各种感染.
- 了解在畜牧环境中的NTM分布对于疾病预防和控制至关重要.
研究的目的:
- 调查牛场和屠宰场环境中的NTM的流行和分布,重点是Mab.
- 评估在这些环境中与NTM相关的潜在动物传染风险.
- 为制定有针对性的干预策略提供流行病学数据.
主要方法:
- 在中国12个省份的畜牧场和屠宰场收集了环境样本.
- 16S rDNA V3-V4区域的高通量测序用于NTM识别和分类.
- 使用定量PCR (qPCR) 来检测和量化Mycobacterium abscessus. 这种细菌.
主要成果:
- 总共分析了1648个环境片.
- 在0.73%的样本中检测到Mycobacterium.
- 在牛场的检测率为0.42% (3/720),在屠宰场的检测率为0.87% (9/928).
结论:
- 包括Mab在内的NTM存在于牛场和屠宰场环境中,表明了潜在的暴露途径.
- 这些发现有助于理解畜牧环境中的NTM流行病学.
- 这项研究支持制定策略,以减轻在畜牧业和公共卫生中与NTM相关的风险.
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