从代谢工程化大肠杆菌中从酸中产生3-基烯酸的高产量生物合成
Wenrui Zhang1, Hongjun Zhang1, Yanzhe Shang2
1State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
从非食品碳来源的酸中*Escherichia coli*进行工程改造,以产生3-基酸 (3-HP). 这种可持续的生物制造方法为工业化工生产提供了具有成本效益的替代方案.
科学领域:
- 生物技术和代谢工程 生物技术和代谢工程
- 可持续化学 可持续化学
- 工业微生物学 工业微生物学
背景情况:
- 第三代生物炼油厂的目标是使用单碳分子和可再生能源进行负碳生产,以应对气候变化和粮食短缺问题.
- 来自合成气发酵的酸盐是大量化学生物合成的有希望的非食品碳来源.
研究的目的:
- 设计大肠杆菌以有效地从乙酸中生产3-基酸 (3-HP).
- 为了优化马洛尼尔-CoA通路,增强3-HP生物合成.
主要方法:
- 在以乙酸为基础的介质中选构成性促进剂,以有效地生产3-HP.
- 抑制脂肪酸合成以减少马洛尼尔-CoA的竞争.
- 辅助因子工程来增强NADPH和乙-CoA的供应.
主要成果:
- 工程菌株ZWR18 ((M*DA) 实现了5.53g/L 3-HP,产量为0.732g/g (97.60%理论).
- 全细胞催化产生了23.89 g/L的3-HP (0.734 g/g,理论上的97.87%).
- 合成气衍生的酸使得ZWR18 ((M*DA) 能够积累18.87 g/L的3-HP (0.58 g/g).
结论:
- 工程设计的大肠杆菌可以通过malonyl-CoA通路从乙酸盐有效地产生3-HP.
- 来自合成气的酸盐是工业生物制造的可行,具有成本效益和可持续的碳来源.
- 这项研究为碳负化学品生产提供了一个有前途的生物炼油途径.
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