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来自Perilla frutescens的细胞外囊泡的抗炎和抗氧化作用:来自斑马鱼模型的见解

Jinghong Huang¹, Linxin Chen², Wenhua Li¹

¹Engineering Research Center of Molecular Medicine of Ministry of Education, Key Laboratory of Fujian Molecular Medicine, Key Laboratory of Xiamen Marine and Gene Drugs, Key Laboratory of Precision Medicine and Molecular Diagnosis of Fujian Universities, Xiamen, Fujian 362021, China; School of Medicine, Huaqiao University, Quanzhou, Fujian 362021, China.

Molecular immunology

|April 23, 2025

概括

来自Perilla frutescens的外体样纳米颗粒 (PELNs) 显示出显著的抗炎和抗氧化作用. 这些来自植物的纳米颗粒是无毒的,并有望作为治疗炎症疾病的治疗剂.

科学领域:

植物生物技术和纳米医学.
自然产品衍生纳米颗粒的表征.

背景情况:

来自植物的细胞外囊泡正在成为具有治疗潜力的强有力的生物活性分子.
来自Perilla frutescens的外体样纳米颗粒 (PELNs) 是一种新型的自然纳米颗粒类.

研究的目的:

研究PELNs的物理化学特性,生物活性作用和治疗潜力.
评估PELNs的抗炎和抗氧化能力.

主要方法:

传输电子显微镜 (TEM) 用于形态和尺寸分析.
多omics和小RNA测序用于货物分析.
在体内斑马鱼模型评估毒性,抗炎和抗氧化性质.
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.
定量实时PCR (qRT-PCR) 和现场杂交用于基因验证.

主要成果:

PELN显示杯状形态,尺寸范围为40-200 nm.
PELNs在斑马鱼中表现出无毒性和减少中性粒细胞迁移,表明抗炎作用.
转录组分析显示,PELNs调节与免疫相关的途径.
通过减少活性氧物种 (ROS) 和改善氧化应激指标,PELNs表现出显著的抗氧化作用.

结论:

PELNs具有显著的抗炎和抗氧化特性.
这些发现凸显了PELNs在治疗炎症和氧化压力相关疾病方面的治疗潜力.
PELNs代表了开发新型植物性治疗剂的有希望的平台.

关键词:

细胞外囊泡细胞外囊泡炎症性炎症性炎症性氧化应激是一种氧化应激.佩里拉果 (Perilla frutescensens) 是一种鲜花的植物.斑马鱼是一种斑马鱼.

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