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解读臭氧对角膜的影响:破坏屏障完整性及其分子驱动因素

Yi Tian¹, Liping Li¹, Zhongmou Sun²

¹Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai 200031, China.

Ecotoxicology and environmental safety

|April 23, 2025

概括

臭氧暴露会破坏小鼠角膜和人类角膜上皮细胞 (HCEC),导致结构变化和激活炎症途径. 酸盐应激和NLRP3炎症酶激活是臭氧诱导的角膜毒性的关键机制.

科学领域:

眼科医生眼科眼科
环境健康环境健康
毒理学毒理学毒理学

背景情况:

臭氧是一种重要的空气污染物,已知对健康有不良影响.
臭氧对眼睛组织,特别是角膜的影响,需要进一步研究以了解细胞和分子反应.

研究的目的:

研究臭氧暴露对小鼠角膜和人类角膜上皮细胞 (HCEC) 的影响.
阐明臭氧诱导的角膜毒性背后的分子机制,重点关注炎症和酸性应激.

主要方法:

鼠标角膜和HCEC暴露于周期性臭氧.
评估角膜结构,上皮屏障功能,细胞活力和炎症标志物.
对酸性压力标志物 (3-酸铁) 和炎症组分 (NLRP3,caspase-1,GSDMD) 的分析.
使用抗化剂 (MnTMPyP) 的治疗干预措施的评估.

主要成果:

暴露于臭氧会导致角膜 stromal 稀薄,上皮质厚度增加和屏障功能受损.
在小鼠角膜中观察到显著的炎症细胞透和3 - 尼托铁素水平升高.
HCECs显示活力降低,酸盐应激增加,以及NLRP3/caspase-1/GSDMD通路的激活.
在HCEC中,MnTMPyP治疗减轻了臭氧诱导的酸盐应激和烧灭.

结论:

暴露于臭氧会通过酸盐应激和NLRP3炎症酶介导的烧灭诱导角膜毒性.
这些发现凸显了臭氧对角膜健康的有害影响.
向酸盐应激和炎症酶激活可能提供针对臭氧相关的角膜损伤的治疗策略.

关键词:

屏障功能屏障功能视角膜视角膜是一个炎症炎症是一种炎症.亚酸盐应激是一种压力.臭氧臭氧是什么?臭氧臭氧是什么

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