在Mythimna separata中,有两条途径调解Cry1Ac的毒性:一条是ABCC2依赖的,另一条涉及ABCC3-CAD相互作用
Hanyue Wang1, Huiran Bian1, Zhenxing Liu2
1Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, School of Life Sciences, Central China Normal University, Wuhan 430070, China.
International journal of biological macromolecules
|April 23, 2025
概括
Mythimna separata的害虫是Bacillus thuringiensis (Bt) 毒素的目标. 研究人员发现,MsABCC3和MsCAD蛋白质共同作用,在这些昆虫中调解Bt毒素的毒性.
科学领域:
- 分子生物学分子生物学
- 昆虫学 昆虫学是一门学科.
- 生物化学 生物化学
背景情况:
- Mythimna separata 是一个重要的农业害虫.
- 已知ATP结合盒 (ABC) 载体可以调节白类昆虫中Bacillus thuringiensis (Bt) Cry1毒素的毒性.
研究的目的:
- 调查MsABCC3在调解Bt毒素毒性的作用.
- 探索MsABCC3,MsABCC2和M. separata对Bt毒素的敏感性中的cadherin (MsCAD) 之间的相互作用.
主要方法:
- 在Hi5昆虫细胞中表达MsABCC3,以评估Cry1Ac毒性.
- 基因编辑创建MsABCC3淘汰M.分离幼虫.
- 在表达 MsABCC3, MsABCC2 和 MsCAD 的 Hi5 细胞中评估 Cry1Ac 细胞毒性.
主要成果:
- MsABCC3调解的Cry1Ac细胞毒性低于MsABCC2,但高于MsCAD.
- MsABCC3和MsCAD的同时表达导致Cry1Ac的敏感性与MsABCC2相似,这表明有协同作用.
- 淘汰的MsABCC3幼虫对Cry1Ac的抗性较低,而单独的MsABCC2则与Cry1Ac的毒性有关.
结论:
- MsABCC3和MsCAD共同作为Cry1Ac的受体.
- 单独的 MsABCC2 会导致 Cry1Ac 的毒性.
- 这些发现为Mythimna separata中Bt毒素抵抗机制提供了新的见解.
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