肥料输入通过调节微生物碳代谢,在土壤中传播了抗生素耐药性基因和毒性因子
Taobing Yu1, Yangkang Huang1, Yicong Zhang1
1State Key Laboratory of Maize Bio-Breeding, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
Environmental pollution (Barking, Essex : 1987)
|April 23, 2025
概括
肥料肥通过增强微生物碳代谢,显著增加土壤中的抗生素耐药性基因 (ARG) 和毒性因子 (VF). 这凸显了碳循环在农业土壤中抗微生物耐药性和病原性传播中的关键作用.
科学领域:
- 环境微生物学 环境微生物学
- 土壤科学 土壤科学
- 农业科学 农业科学
背景情况:
- 土壤中的抗生素耐药性基因 (ARG) 和毒性因子 (VF) 构成重大生态和人类健康风险.
- 肥料肥料产生富含碳的土壤,可能会影响微生物群落和ARG和VF的扩散.
- 长期施肥和灌对微生物碳代谢的影响及其与农业系统中ARG/VF的联系仍然不太清楚.
研究的目的:
- 研究长期施肥和灌对土壤微生物碳代谢,ARG和VF分布的影响.
- 确定微生物碳代谢,社区组成和ARG和VFs的丰度之间的关系.
- 阐明微生物碳代谢在推动小麦-玉米系统中ARG和VF传播中的作用.
主要方法:
- 实地实验:在小麦生长期间进行了9年的不同施肥 ( vs. NPK vs. none) 和灌处理.
- 分析土壤微生物的碳代谢,ARG和VF的丰度和类型,以及微生物群落的组成.
- 包括部分最小平方路径建模和相关性在内的统计分析,以评估变量之间的关系.
主要成果:
- 与没有肥料和NPK肥料处理相比,肥料应用显著增加了ARG和VF的总丰度和类型.
- 肥料肥料丰富了参与碳固定和降解的微生物 (例如,Streptomyces,Lysobacter,Agromyces) 以及增加的碳水化合物活性酶 (CAZymes) 和碳循环途径.
- 微生物多样性,CAZymes,碳循环路径 (固定和降解) 和整体碳代谢能力与ARG和VF增殖有正相关.
结论:
- 在农业土壤中长期施加肥料有助于ARG和VF的扩散和传播.
- 微生物的碳代谢,特别是碳固定和降解途径,在调解ARG和VF传播方面发挥着至关重要的作用.
- 了解微生物碳代谢对于管理密集农业实践下的土壤中抗微生物耐药性和病原性至关重要.
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