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通过绿色技术处理的果废物中的5-基甲基黄衍生物的全酵母细胞合成

Cristiana Martins¹, Carlos E Costa², Joana T Cunha³

¹Departamento de Enxeñaría Química, Universidade de Vigo, Facultade de Ciencias, Ourense 32004, Spain; Instituto de Agroecoloxía e Alimentación, Universidade de Vigo - Campus Auga, Ourense 32004, Spain.

Bioresource technology

|April 23, 2025

概括

这项研究将果果渣废物转化为有价值的化学物质,如furandicarboxylic acid (FDCA),使用深度溶解剂和微波加热. 工程酵母然后有效地生产FDCA和其他衍生品,提供可持续的生物炼油解决方案.

科学领域:

生物技术是生物技术.
绿色化学绿色化学
可持续材料可持续材料

背景情况:

气候变化需要转向可再生资源和生物工艺.
生物炼油厂可以利用农业工业废物,如果果汁,制成有价值的产品.
可持续的化学品生产对于减少环境影响至关重要.

研究的目的:

开发一种高效的工艺,从果果汁中生产5-基甲基 (HMF) 衍生物.
在HMF生产中使用深度环氧溶剂 (DES) 和微波辅助加热.
采用工程化Saccharomyces cerevisiae用于将HMF生物转化为FDCA等高价值化学品.

主要方法:

果残渣的水解以获得HMF.
对于催化HMF生产而言,深度欧溶剂 (DES) 和微波辅助加热.
使用表达HMF氧化酶的工程Saccharomyces cerevisiae菌株进行HMF生物转化.

主要成果:

在最佳条件下 (160°C,30分钟,50%的胆化物:乳酸DES) 产生了34.6%的HMF.
工程酵母菌株将HMF转化为FDCA和FFCA.
最好的菌株产生了10.8毫米的FDCA和15.5毫米的FFCA.

结论:

结合微波助力DES技术和微生物生物催化剂的综合方法使农业废物有效的回收利用成为可能.
这个过程为从副产品中生产可再生化学品提供了一个可持续的途径.
该研究强调了生物炼油厂在创造循环经济方面的潜力.

关键词:

深层欧性溶剂深层欧性溶剂酶性水解是一种酶性水解.代谢工程是代谢工程.微波炉微波炉微波炉微波炉微波炉微波炉微波炉微波炉微波炉微波炉微波全细胞生物转化全细胞生物转化

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