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可扩展的超极化MRI,通过[1-13C]Pyruvate的Ace-SABRE实现

Stephen J McBride¹, Megan Pike², Erica Curran¹

¹North Carolina State University, Department of Chemistry, UNITED STATES OF AMERICA.

Angewandte Chemie (International ed. in English)

|April 23, 2025

概括

通过可逆交换信号放大 (SABRE) 超极化[1-13C]pyruvate使用乙-水溶剂. 这种方法为体内代谢成像产生可注射溶液,并为传统技术提供了更简单的替代方案.

科学领域:

磁共振成像是一种磁共振成像技术.
分子成像学分子成像学
生物化学生物化学

背景情况:

使用[1-13C]pyruvate的高极化 (HP) MRI是一种有前途的分子成像技术.
通过可逆交换信号放大 (SABRE) 在溶液中提供直接基板极化,避免复杂的硬件.
传统的SABRE方法通常使用甲醇,一种很难去除的有毒溶剂.

研究的目的:

开发和验证一种乙-水溶剂系统 (Ace-SABRE) 用于高极化[1-13C]酸盐生产.
使用Ace-SABRE.使用超极化[1-13C]pyruvate制造可注射溶液.
证明Ace-SABRE在体内代谢跟踪和MRI中的实用性,将其与溶解动态核极化 (d-DNP) 进行比较.

主要方法:

使用80/20的乙-水溶剂混合物进行SABRE超极化[1-13C]pyruvate.
开发了一种溶剂处理协议,以获得可注射的超极化[1-13C]酸盐溶液.
在肝细胞癌 (HCC) 瘤模型上使用Ace-SABRE平台进行体内光谱和MRI.

主要成果:

使用Ace-SABRE系统实现了[1-13C]pyruvate的极化率高达17%.
在溶剂处理后,最终注射溶液中保留了74%的初始偏振.
在HCC瘤中成功证明了代谢跟踪和HP-MRI,与d-DNP结果相比.

结论:

艾斯-SABRE技术为生产高极化[1-13C]pyruvate.提供了一个可行的,少有毒的替代方案.
这种方法简化了生物实验的过程,降低了HP-NMR和HP-MRI的进入障碍.
在临床前和临床分子成像应用中,Ace-SABRE显示出更广泛采用的巨大潜力.

关键词:

过极化的超极化磁共振成像 (MRI) 的使用.代谢成像 - - 代谢成像准气为一种气.皮鲁酸是一种酸盐.

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