相关实验视频
Updated: Jun 19, 2026
10:19
Derivation of Mouse Trophoblast Stem Cells from Blastocysts
Published on: June 8, 2010
18.1K
α-二甲酸促进了来自原始人类胚胎干细胞的托菲克托德的诱导和成熟
Karlien Van Nerum1, Anne Wenzel1, Lidia Argemi-Muntadas2
1Novo Nordisk Foundation Center for Stem Cell Medicine - reNEW, Department of Biomedical Sciences, Faculty of Health and Medical Science, University of Copenhagen, Copenhagen, Denmark.
Nature cell biology
|April 24, 2025
概括
代谢会影响细胞的发育. 阿尔法-谷酸盐 (αKG) 积累有助于通过改变染色体调节来改变人体干细胞体干细胞谱系的规范和成熟.
科学领域:
- 发展生物学 发展生物学
- 代谢调节 代谢调节 代谢调节 代谢调节
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 细胞状态转换涉及复杂的转录,表观遗传和代谢过程的相互作用.
- 特定的代谢物,如α-谷氨酸 (αKG),作为信号分子,影响染色素修饰酶.
- 在人类植入前发育过程中,新陈代谢在协调细胞状态变化的确切作用尚不完全理解.
研究的目的:
- 为了研究在人类胚胎干细胞中在托菲克托德尔玛系系诱导过程中的代谢重新连接.
- 阐明alpha-ketoglutarate (αKG) 在促进皮的命运规范和成熟中的作用.
- 了解代谢变化如何调节早期人类发育期间的染色质动态和多能性.
主要方法:
- 诱导天真的人类胚胎干细胞向 trofhectoderm 血统.
- 代谢分析,以确定关键代谢物变化,特别是α-谷氨酸 (αKG) 积累.
- 评估布拉斯极化和 trofhectoderm 成熟标志物.
- 分析基因组甲基化和乙化水平,包括乙-CoA的可用性和基因组乙转移酶活性.
主要成果:
- 在人类胚胎干细胞中诱导托菲克托德尔姆系的诱导与显著的α-谷酸盐 (αKG) 积累有关.
- 升高的αKG水平增强了托菲克多德的特异化能力,并促进了布拉斯极化和成熟.
- αKG补充剂没有改变全球基因素甲基化,但降低了乙-CoA,降低了基因素乙转移酶活性,并削弱了多能性网络.
结论:
- 代谢作为一个积极的反机制,支持托菲克多德的命运诱导和成熟.
- 全球代谢重新连接,以αKG积累为例,增强了细胞命运决定的特异性.
- 信号和色素动态的新陈代谢调节对于人类植入前胚胎的 trofhectoderm 发育至关重要.
相关概念视频
01:38Fertilization
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
01:33Cleavage and Blastulation
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
01:56Gastrulation
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...
01:10Zygotic Development And Stem Cell Formation
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
00:57Cellular Differentiation
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
A zygote is a...
01:07Teratogenicity
The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...