相关实验视频

Updated: May 12, 2025

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Published on: July 23, 2014

编码desUMOylating异酶的ZmDeSI2促进体中的自然变异控制了玉米中核甲基因含量

Xin Lu¹, Yuhong Lei¹, Zhennan Xu¹

¹State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

Molecular plant

|April 24, 2025

概括

研究人员确定了一种关键基因ZmDeSI2,该基因控制了玉米核中的氨酸水平. 一种特定的ZmDeSI2变体显著增加了氨酸含量,通过玉米生物强化为改善动物料提供了一个有希望的途径.

科学领域:

植物遗传学植物遗传学
分子生物学分子生物学
营养生物化学营养生物化学

背景情况:

氨酸 (Met) 是动物料中至关重要的必需氨基酸,但其在玉米中的低含量限制了其营养价值.
控制玉米核中的Met积累的遗传和分子基础尚不清楚.

研究的目的:

调查玉米核中Met含量调节的基因和分子机制.
为了确定可以提高Met水平以改善动物营养的遗传变异.

主要方法:

全基因组关联研究 (GWAS) 在348个杂交玉米系.
转录组分析以确定关键基因.
生物化学实验以阐明基因功能.
标记器辅助育种产生修饰的近同位素线.

主要成果:

鉴定了deSUMOylating异酶基因ZmDeSI2作为Met含量的关键调节者.
ZmDeSI2直接降低硫酸盐还原酶 (ZmSIR) 的活性,从而抑制甲生物合成.
ZmDeSI2促进体的自然变异,特别是ZmWRKY105结合部位的存在或不存在,决定了ZmDeSI2的表达水平.
一个精英的ZmDeSI2促销器哈普类型 (ZmDeSI2^Hap2) 缺乏结合点,导致内核Met含量增加1.36倍.

结论:

通过调节ZmSIR,ZmDeSI2在控制玉米核中的Met积累方面发挥着至关重要的作用.
ZmDeSI2^Hap2单种代表了一种有价值的遗传资源,用于提高玉米育种计划中的氨酸生物强化.
这项研究为为动物料行业开发改进的玉米品种提供了分子基础.

关键词:

脱SUMOylation 脱SUMOylation 脱SUMOylation 脱SUMOylation 脱SUMOylation 脱SUMOylation 脱SUMOylation 脱SUMOylation 脱SUMOylation 脱SUMOylation 脱SUMOylation 脱SUMOylation 脱SUMOylation 脱SUMOylation 玉米玉米玉米是一种甲氨酸的使用方法自然变化的自然变化.硫酸盐还原酶可以减少硫酸盐.

更多相关视频

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves

Published on: December 2, 2016

相关概念视频

Position-effect Variegation

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.

Dihybrid Crosses

Dihybrid Crosses

The Eukaryotic Promoter Region

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...