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在MEcPP中介的热耐受性和生长调节中,HDS和HSFA1功能的整合在Physcomitrium patens中

Xiaojun Pu^1,2,3, Zhuo Yang¹, Yongjun Lin⁴

¹State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, People's Republic of China.

Plant, cell & environment

|April 24, 2025

概括

菲斯科米特里姆病原体 HDS 基因家族扩张有助于耐热性. HDS与HSFA1相互作用,通过调节甲基酸路径中间体来调节应激反应和生长.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
进化生物学进化生物学

背景情况:

陆地植物具有应激适应机制.
甲基酸 (MEP) 途径的2-C-甲基-D-酸2,4-环二酸 (MEcPP) 对于叶绿体逆行信号和压力反应至关重要.
在非血管植物中,MEcPP的规范性尚不明朗.

研究的目的:

描述Physcomitrium patens中的1-hydroxy-2-methyl-2-(E) -butenyl-4-diphosphate合成酶 (HDS) 基因家族的特征.
研究HDS在耐热性中的作用及其与热冲击转录因子A1 (HSFA1) 的相互作用.
探索 HDS 在植物中的进化模式.

主要方法:

在Physcomitrium patens中基因家族的表征.
基因编辑CRISPR/LbCas12a以创建HDS突变的基因编辑.
MEcPP水平的量化和外部应用.
转录基因分析.转录基因分析.
HSFA1相互作用研究.

主要成果:

菲斯科米特里姆病原体拥有多个HDS副本,与大多数血管精子不同.
HDS突变体表现出增强的耐热性和升高的MEcPP水平.
HDS与HSFA1相互作用,调节热耐受性和应激反应基因表达.
HDS和HSFA1协同调节植物生长,IAA水平和非光化学火.

结论:

在Physcomitrium patens中发现了一种新的HDS-HSFA1调节模块.
该模块扩展了叶绿体逆行信号范式,并增强了应激适应.
研究结果提供了对植物中HDS功能演变和应激耐受性的见解.

关键词:

在 HDS 和 HDS 之间.这是一个MEcPP.叶绿体的逆行信号传输系统发展发展发展发展发展.热冲击因子 HSFA1 热冲击因子 HSFA1 热应激是一种热应激.

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