益生菌通过调节PI3K和TGF-β/Smad信号通路来促进细胞的伤口愈合反应
Sixuan Zhang1, Yvonne Elbs-Glatz1, Siyuan Tao1
1Empa, Swiss Federal Laboratories for Materials Science and Technology, Biointerfaces Lab, St. Gallen, 9014, Switzerland.
Cell communication and signaling : CCS
|April 24, 2025
概括
益生菌BioK通过调节基因表达和pH来增强人类皮肤纤维细胞迁移,并减少纤维化标志物. 这表明益生菌可以改善伤口愈合,并最大限度地减少痕形成.
科学领域:
- 伤口愈合研究研究研究
- 细胞生物学 细胞生物学
- 益生菌的应用 益生菌的应用
背景情况:
- 皮肤伤口愈合是一个复杂的过程,涉及纤维细胞迁移和纤维化调节.
- 纤维细胞活动对于伤口关闭和组织修复至关重要.
- 益生菌治疗是一种新兴的策略,用于调节伤口愈合和纤维化.
研究的目的:
- 研究细菌益生菌 (BioK) 对人类皮肤纤维细胞 (HDF) 迁移的影响.
- 评估BioK在HDF中的抗纤维性潜力.
- 探索乳酸在BioK介导对纤维细胞活动的影响中的作用.
主要方法:
- 在体外,BioK与HDF的共同培养.
- 转录组分析和RT-qPCR用于基因表达.
- 对于蛋白质水平评估的ELISA.
- 检查乳酸在pH介导作用中的作用.
主要成果:
- 生物基因显著促进了HDF迁移,与PI3K通路基因 (Paxillin,PI3K,PKC,ITG-β1) 的上调相关.
- 生物K下调了TGF-Smad通路基因 (Nox-4,α-SMA,Col-I),减少了肌纤维细胞分化和原蛋白的产生.
- 由BioK生产的乳酸被确定为pH调节和纤维细胞活性的关键因素.
结论:
- 生物K证明对纤维细胞迁移有有益影响,并具有抗纤维细胞性质.
- 度调节和特定基因调节是BioK作用的关键机制.
- 像BioK这样的益生菌为减少伤口愈合中的痕形成提供了有前途的治疗潜力.
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