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基准剂量估计从转录学数据的甲基胺离子液体肝毒性:对健康风险评估的绿色溶剂的影响

Qing Yang¹, Xiaole Zhao¹, Kejia Wu²

¹College of Food Science and Engineering, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China.

Environment & health (Washington, D.C.)

|April 24, 2025

概括

像M8OI这样的离子液体 (IL) 显示有毒性. 转录学和剂量反应分析揭示了M8OI的分子机制和敏感的毒性值,有助于安全评估.

科学领域:

环境毒理学环境毒理学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

离子液体 (ILs) 越来越多地使用,但存在潜在的环境风险.
1-octyl-3-methylimidazolium (M8OI),一种IL,具有肝毒性,在土壤中发现.
了解M8OI的分子毒性机制对于安全性评估至关重要.

研究的目的:

为了阐明M8OI诱导的肝毒性分子机制.
通过剂量反应分析得出敏感的转录基因起点 (tPOD).
为了将tPOD与已知的M8OI肝毒度进行比较.

主要方法:

使用全转录组测序来分析基因表达变化.
基准剂量 (BMD) 建模用于确定转录基因起点 (tPOD).
对暴露于M8OI的生物体的基因表达数据进行了剂量反应分析.

主要成果:

在低剂量,中剂量和高剂量的M8OI中观察到显著的基因表达差异.
鉴定出了细胞因子-细胞因子受体相互作用和IL-17信号通路的干扰.
转录学衍生的tPODs比M8OI的肝毒性EC50.0显著低 (至少是105倍).

结论:

M8OI通过特定的分子途径诱导肝毒性,包括免疫信号.
转录组学与BMD建模相结合,提供了敏感和定量毒性值.
这些发现支持改善了对IL安全性的毒理学评估和监管评估.

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