用酶固定的基于氧化石墨烯的电化学生物传感器来检测谷氨
Neeta Ukirade1,2, Upasana Choudhari3, Sunil Bhapkar4
1Centre for Materials for Electronics Technology (C-MET) Panchawati, Off. Dr Bhabha Road Pune-411008 India sunitrane@yahoo.com.
RSC advances
|April 24, 2025
概括
在石墨烯氧化物上使用乳酶酶的新型电化学传感器检测出一种关键抗氧化剂谷氨. 这种传感器显示出高灵敏度和稳定性,用于监测真实样本中的氧化应激标记物.
科学领域:
- 生物医学工程 生物医学工程
- 电化学 电化学 电化学
- 材料科学 材料科学 材料科学
背景情况:
- 谷氨是一种重要的抗氧化剂,其耗尽表明氧化应激.
- 开发敏感的谷氨检测方法对于健康监测至关重要.
研究的目的:
- 开发一种敏感的电化学传感器来检测谷氨.
- 为了利用在石墨烯氧化物 (GO) 上固定的乳酶,提高传感能力.
主要方法:
- 氧化 (ITO) 电极表面通过滴滴造用GO进行修改.
- 在GO修改的ITO表面上固定乳酶酶.
- 使用FESEM,XPS,FTIR,CV和EIS进行表征;用电化学检测谷氨 (GSH).
主要成果:
- FTIR,FESEM和拉曼分析证实GO上的laccase固定成功.
- 乳糖酶/GO/ITO电极表现出对GSH氧化增强的电催化活性.
- 实现了广泛的线性范围 (1 μM100 μM),低检测极限 (0.89 μM) 和高灵敏度 (6.51 μA μM-1).
结论:
- 开发的电化学传感器提供高灵敏度,选择性,可重复性和稳定性来检测谷氨.
- 传感器在真实样本中成功检测到GSH,证明了其实际应用.
- 这种方法提供了一个有前途的工具,通过谷氨水平监测来评估氧化应激.
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