肠道细菌,免疫系统和Plutella xylostella中的Cry1Ac毒素之间的战斗
Wenyu Liao1, Zhuohong Lv1, Yunfei Mo1
1Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha 410128, China.
Journal of agricultural and food chemistry
|April 24, 2025
概括
肠道细菌会影响昆虫对Bacillus thuringiensis (Bt) Cry1Ac毒素的敏感性. 一种Enterobacter菌株通过降解毒素来保护昆虫,而另一种则增加易感性,导致败血症.
科学领域:
- 微生物生态学 微生物生态学
- 昆虫病理学 昆虫病理学
- 分子毒理学 分子毒理学
背景情况:
- 细菌 thuringiensis (Bt) 哭毒素是广泛使用的生物杀虫剂.
- 关于Bt-pest相互作用的研究一直专注于毒素受体,忽视了肠道微生物群的作用.
- 肠道微生物群对昆虫对Bt毒素敏感性的影响仍未得到充分研究.
研究的目的:
- 研究肠道细菌在调节昆虫对Cry1Ac毒素敏感性的作用.
- 为了识别特定的细菌菌株及其反应对Cry1Ac暴露的作用机制.
主要方法:
- 从昆虫肠道微生物群中分离和识别Enterobacter菌株.
- 评估细菌菌株对Cry1Ac原蛋白降解的影响.
- 通过qPCR分析昆虫免疫通路激活 (proPO-AS,JNK).
- 评估Cry1Ac治疗后的昆虫生存率和血淋巴细菌入侵.
主要成果:
- 肠杆菌hormaechei (PxG15) 降解了Cry1Ac原蛋白,激活了proPO-AS和JNK通路,减少了中肠损伤,并防止了血淋巴的入侵.
- 肠杆菌asburiae (PxG1) 显示较慢,较弱的JNK通路激活,损害中肠修复,并导致由于迅速的血淋巴入侵导致败血症.
- 不同的肠道细菌活动显著影响Cry1Ac暴露下昆虫的生存.
结论:
- 肠道细菌在确定昆虫对Bacillus thuringiensis Cry1Ac.敏感性方面发挥着至关重要的作用.
- 肠杆菌菌株通过涉及毒素降解和免疫调节的独特机制对Cry1Ac毒性表现出相反的影响.
- 了解这些宿主-微生物-毒素相互作用为害虫控制提供了新的策略.
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