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Updated: May 10, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

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深度突变扫描和CRISPR工程病毒:进化和功能基因组学研究的工具.

Mercedes Paz1,2, Gonzalo Moratorio1,2,3

  • 1Laboratory of Experimental Virus Evolution, Institut Pasteur de Montevideo, Montevideo, Uruguay.

mSphere
|April 24, 2025
PubMed
概括

合成生物学和测序推进了病毒基因组操纵. 深度突变扫描和CRISPR基因组编辑增强了对病毒进化的理解,有助于未来的疫苗和治疗策略.

关键词:
使用CRISPR工程的病毒.深度突变扫描 (deep mutational scanning) 是一种对突变进行深度扫描的方法.病毒的演变 病毒的演变

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相关概念视频

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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