对微生物检测的元基因组cfDNA测序进行全面评估
Zhenli Diao1,2, Zihong Zhao1,3,4, Yanxi Han1,3
1National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, Beijing, P. R. China.
Clinical chemistry
|April 24, 2025
概括
甲基因组无细胞DNA (cfDNA) 测序显示了传染病诊断的高准确性. 确定了优化病原体检测和提高诊断准确性的关键因素.
科学领域:
- 临床诊断 临床诊断 临床诊断
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 超基因组无细胞DNA (cfDNA) 测序为诊断传染病提供了一种新的方法.
- 准确和可比的微生物cfDNA测序存在挑战,原因是低度,碎片化和方法复杂性.
- 这项研究评估了性能,并确定了影响甲基因组cfDNA测序精度的因素,用于在血中检测微生物.
研究的目的:
- 评估多个实验室的元基因组cfDNA测序的性能,以检测血中的微生物.
- 确定影响元基因组cfDNA测序准确性的因素.
- 为这种诊断技术的开发和优化提供指导.
主要方法:
- 使用了18个微生物cfDNA社区的参考小组.
- 在130个实验室中评估了性能,评估了准确性,可重复性,抗干扰性,检测极限 (LoD) 和线性相关性.
- 分析了污染源 (湿实验室与干实验室) 和干扰因素.
主要成果:
- 大多数实验室表现出色,平均F1得分为0.98.
- 污染主要源于湿实验室,而干实验室是假负误差的主要来源.
- 可复制性,LoD和线性是有利的;人类cfDNA的干扰是最小的,但基因相似的微生物导致更明显的干扰.
- 病毒cfDNA检测性能低于细菌和真菌检测.
结论:
- 建立了超基因组cfDNA测序的现实世界性能.
- 确定了影响准确性和优化的关键因素,为临床应用提供了指导.
- 这些发现支持采用元基因组cfDNA测序来准确检测传染病中的病原体.
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