生杆菌腹 sp. 在这里. Nov. 从物仓鼠中分离出来的皮下
Tsuyoshi Nakahara1, Akihiro Nakamura2, Kiyoko Tamai1
1Department of Clinical Laboratory, Miroku Medical Laboratory Inc., Saku, Japan.
概括
新的与仓鼠相关的细菌, *Rodentibacter abscessus* sp. sp. 这种细菌 十一月,被确定了. 这些新型菌株与其他 *Rodentibacter* 菌种有所区别,基于遗传和生化分析.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 动物传播疾病 动物传播疾病
背景情况:
- 属 *Rodentibacter *包括家族 *Pasteurellaceae *中的细菌,已知与各种动物宿主有关.
- 了解虫菌物种的多样性和特征对于动物健康和潜在的公共健康至关重要,因为一些物种可以成为机会性病原体.
研究的目的:
- 在日本从物仓鼠中分离出来的五种细菌菌株的特征.
- 根据基因型和表型分析,确定这些隔离物是否代表Rodentibacter*属内的新物种.
主要方法:
- 基因组分析包括16S rRNA基因测序,平均核酸标识 (ANI) 和数字DNA-DNA杂交 (dDDH).
- 用生物化学测试 (如血液溶解) 和酶活性测试 (例如α-葡萄糖酶,β-葡萄糖酶) 来进行表型表征.
- 将基因型和表型数据与已知的 *Rodentibacter* 种类进行比较,特别是 *Rodentibacter haemolyticus*.
主要成果:
- 五个与仓鼠相关的分离物体在基因型和表型上与已知的Rodentibacter物种不同.
- 与 *Rodentibacter haemolyticus* 的遗传相似性表明了96.5%的16S rRNA基因序列相似性,90.8%的ANI和42.7%的dDDH.
- 新型菌株表现出独特的生物化学特征,包括对α-葡萄糖酶,l-阿拉比诺酸和三酸的正反应,对β-葡萄糖酶,曼诺酸,伊诺西托和糖醇的负反应,使它们与R. haemolyticus*不同.
结论:
- 独特的基因型和表型特征支持将这些分离物种归类为Rodentibacter*属内的新物种.
- 这种新物种的拟议名称是 *Rodentibacter abscessus* sp. 在新的一年里.
- 类型菌株THUN1654T已存储在国际种植集中,以供未来的参考和研究.
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