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库尔库通过调节AMPK/mTOR介导的自改性来改善因衰老引起的血屏障破坏
Chen Xue1,2, Zhenxing Yan1,2, Wenjing Cheng1,2
1College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China.
PloS one
|April 24, 2025
概括
黄素通过恢复塞尔托利细胞的紧密结节 (TJ) 来保护血液丸屏障 (BTB) 免受衰老损伤. 这种效应通过AMPK/mTOR通路激活自,为治疗男性生殖障碍提供了潜力.
科学领域:
- 生殖生物学 生殖生物学
- 细胞衰老 细胞衰老
- 药理学 药理学是指药理学的学科.
背景情况:
- 血屏障 (BTB),由塞尔托利细胞 (SCs) 之间的紧密连接 (TJ) 形成,对精子生成至关重要.
- 衰老会损害BTB的完整性,导致丸功能障碍.
- 黄素对丸中与年龄相关的TJ损伤的影响尚不清楚.
研究的目的:
- 为了研究黄素对D-银糖诱导的老化损伤在老鼠丸SCs的保护作用.
- 阐明黄素对TJ完整性和自作用的分子机制.
- 探索AMPK/mTOR途径在黄素治疗效果中的作用.
主要方法:
- 在体内 (老鼠丸) 和体内 (TM4细胞) 已建立的D-银糖诱导衰老模型.
- 管理的黄素和评估的TJ蛋白表达 (ZO-1,Claudin-4,Claudin-7,Occludin) 和衰老标志物 (p16,p21).
- 研究了自水平和自调节剂 (CQ,RAPA) 和AMPK/mTOR通路的参与.
主要成果:
- D-银糖诱导衰老,TJ蛋白减少,并减少了自.
- 黄素逆转了D-银糖诱导的TJ损伤,并恢复了自水平.
- 由AMPK/mTOR途径调解的自活化对黄素的保护作用至关重要.
结论:
- 黄素通过增强丸SCs中的TJ完整性来改善因衰老引起的BTB损伤.
- 保护机制包括通过AMPK/mTOR信号通路的黄素诱导的自细胞激活.
- 黄素对与年龄相关的男性生殖系统疾病具有潜在的治疗策略.
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