基于 ω-3 脂肪酸的结构化脂的酶生物合成,表征和抗氧化特性
Yuxin Wu1, Pengkai Xie1, Ning Zhang1
1China-Malaysia Belt and Road Joint Laboratory on Oil Processing and Safety, Jinan University, China.
Food chemistry
|April 24, 2025
概括
用酶方法合成富含omega-3脂肪酸 (ω-3-PLs) 的结构化脂. 这些 ω-3-PLs 显示出更好的稳定性和抗氧化特性,可用于食品和补充剂中的潜在用途.
科学领域:
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
- 营养保健品 营养保健品
背景情况:
- 欧米茄-3脂肪酸对生理功能至关重要.
- 商用欧米茄-3乙烯 Ester 的生物可用性和稳定性都低于最佳.
- 与脂结合的omega-3脂肪酸提供了卓越的生物利用性和稳定性.
研究的目的:
- 合成富含omega-3脂肪酸 (ω-3-PLs) 的结构化脂.
- 为 ω-3-PL 合成使用无溶剂的酶方法.
- 评估合成的 ω-3-PLs 的特性,抗氧化活性和氧化稳定性.
主要方法:
- 用脂酶A1 (PLA1) 与大豆脂酶对藻类油 (富含 ω-3 脂肪酸) 的酶性兴趣化.
- 反应条件的优化:基质质量比 (1:8 w/w),水含量 (0.4%),酶负荷 (2.5%),温度 (65°C) 和反应时间 (12小时).
- 使用31P NMR,GC-FID,UV,XRD,FT-IR和TGA进行表征;评估抗氧化活性和氧化稳定性.
主要成果:
- 在优化条件下, ω-3 脂肪酸在脂中实现了 41.54% 的合并率.
- 合成的 ω-3-PLs 显示出增强的氧化稳定性.
- 证明了 ω-3-PLs 的显著抗氧化潜力.
结论:
- 无溶剂的酶合成成功产生了具有改进性质的 ω-3-PLs.
- 合成的 ω-3-PLs 具有增强的氧化稳定性和抗氧化能力.
- 这些发现支持 ω-3-PLs 在功能性食品和营养保健品中的应用.
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