相关实验视频

Updated: May 20, 2025

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

癌症干细胞和与瘤相关的巨细胞:相互作用和治疗机会

Haitao Yuan¹, Yun Qiu¹, Zijie Mei¹

¹School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, PR China.

|April 24, 2025

概括

癌症干细胞 (CSCs) 在瘤微环境 (TME) 中招募和重新编程单细胞成为与瘤相关的巨细胞 (TAM). 然后TAM增强CSC干和瘤进展,突出了关键的治疗标.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

癌症干细胞 (CSC) 依赖瘤微环境 (TME) 进行生存和自我更新.
单细胞被招募到TME,并分化为与瘤相关的巨细胞 (TAMs).
CSCs和TAMs之间的相互作用对瘤进展和治疗耐药性至关重要.

研究的目的:

审查癌症干细胞 (CSCs) 和瘤相关巨细胞 (TAMs) 之间的复杂相互作用.
阐明CSCs影响单细胞招募和TAM两极化的机制.
探索针对CSC-TAM轴的治疗策略,以改善癌症治疗.

主要方法:

对调查CSC-TAM交叉通话的研究进行文献综述.
通过CSC驱动单细胞招募和TAM偏振的分子机制的分析.
检查针对癌症TAM的治疗干预措施.

主要成果:

CSCs积极招募单细胞并促进它们分化为TAMs.
通过分泌因子和直接相互作用,TAMs增强CSC茎度,利基维护和瘤生长.
TAMs通过化学因子,细胞因子,外体介导的miRNA转移和细胞外基质重塑来促进瘤的进展.

结论:

中枢细胞-TAM轴代表了瘤进展和治疗耐药性的关键驱动因素.
通过差异化抑制,极化重编程或免疫疗法向TAM提供了有前途的治疗途径.
调节CSC-TAM相互作用有可能提高当前癌症治疗的疗效.

关键词:

通过CAR-巨治疗治疗.癌症干细胞是癌症干细胞.癌症干的维护癌症干的维护化学基因/细胞基因信号传递外体的miRNAs可以使用.免疫检查站封锁免疫检查站封锁巨细胞的两极分化瘤微环境是一个微环境.与瘤相关的巨细胞.

更多相关视频

In Vitro Assay to Study Tumor-macrophage Interaction

In Vitro Assay to Study Tumor-macrophage Interaction

Published on: August 1, 2019

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Published on: October 20, 2016

相关概念视频

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

Cancer Stem Cells and Tumor Maintenance

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

Adaptive Mechanisms in Cancer Cells

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

Metastasis

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...