相关实验视频
Updated: Jul 11, 2026

17:14
In vivo and in vitro Studies of Adaptor-clathrin Interaction
Published on: January 26, 2011
14.4K
miR398-SlCSD1模块参与了Solanum lycopersicum中的SA-H2O2放大反循环
Xiujuan Wang1, Xinshan Zhang1, Yuanyuan Liu2
1College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300 Zhejiang, China.
Journal of advanced research
|April 24, 2025
概括
这项研究揭示了miR398-SlCSD1模块通过调节活性氧物种 (ROS) 来放大番茄中酸 (SA) 信号传输. 这揭示了一个新的反循环,对植物免疫力和耐压力至关重要.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物信号通路 植物信号通路
- 植物免疫力 植物免疫力
背景情况:
- 酸 (SA) 是植物免疫反应中的关键植物激素.
- 据认为,SA信号由活性氧物种 (ROS) 放大,但机制尚不清楚.
- 了解SA-ROS相互作用对于植物的压力耐受性至关重要.
研究的目的:
- 研究miR398b-SlCSD1模块在番茄 (Solanum lycopersicum) 中的SA-H2O2放大反循环中的作用.
- 阐明植物免疫中SA自我放大背后的分子机制.
主要方法:
- 在不同SA度下评估ROS代谢和基因表达 (SlCSD1,sly-miR398).
- 使用CRISPR/Cas9进行sly-miR398和SlCSD1.1的基因淘汰.
- 采用生物信息学,双露西法酶报告员测定和EMSA来识别监管相互作用.
主要成果:
- 低SA度诱导H2O2的积累,并抑制了sly-miR398的表达.
- SlCSD1淘汰赛部分抑制了SA诱导的H2O2积累,证实了它在SA-ROS信号传递中的作用.
- TGA2被确定为一种转录因子,它调解了miR398-SlCSD1模块的SA调节.
- 通过PAL和ICS途径,sly-miR398b的过度表达/突变促进了SA合成.
结论:
- miR398-SlCSD1模块参与番茄中的SA-H2O2放大反循环.
- 这项研究为SA信号和SA-ROS相互作用提供了新的见解.
- 针对microRNA调节的途径提供了提高作物应激耐受性的潜力.
相关概念视频
Amplifying Signals via Enzymatic Cascade
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
Cell Signaling Feedback Loops
Positive and negative feedback loops are crucial for regulating biological signaling systems. These feedback loops are processes that connect output signals to their inputs.
Negative feedback loops
Most signaling systems have negative feedback loops that can perform different functions such as output limiter, and adaptation.
Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...
Negative feedback loops
Most signaling systems have negative feedback loops that can perform different functions such as output limiter, and adaptation.
Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...
MOSFET Amplifiers
The MOSFET, when operating in its active region, functions as a voltage-controlled current source. In this region, the gate-to-source voltage controls the drain current. This principle underlies the operation of the transconductance MOSFET amplifier. The output current is directed through a load resistor to convert this amplifier into a voltage amplifier. The output voltage is then obtained by subtracting the voltage drop across the load resistance from the supply voltage. This process results...
Microbial Fuel Cells
Microbial fuel cells (MFCs) are bioelectrochemical devices that generate electricity by exploiting the metabolic processes of electrogenic bacteria. These systems provide a renewable energy source and serve as an innovative method for treating organic waste, such as wastewater.A typical MFC consists of two chambers: an anoxic (oxygen-free) compartment that houses the bacteria and an oxic (oxygen-rich) compartment that contains oxygen as the terminal electron acceptor. Many MFCs use proton...
Bioreactor Controls-II
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Microbial Biosensors
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

