相关实验视频

Updated: May 10, 2025

Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients

Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients

Published on: June 11, 2011

肠道微生物群在不同免疫反应中的表征在PLWH中

Yanyan Guo¹, Gan Tang^2,3, Ziwei Wang^2,3

¹Chuzhou Center for Disease Control and Prevention, Chuzhou, 239000, Anhui, China.

Scientific reports

|April 24, 2025

概括

肠道微生物失调与接受抗逆转录病毒疗法 (ART) 的艾滋病毒感染者 (PLWH) 的各种反应有关. 特定细菌的变化与免疫反应和炎症标志物相关,这表明它们在治疗结果中起作用.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
传染性疾病传染性疾病

背景情况:

在艾滋病毒感染者 (PLWH) 中记录了肠道微生物失调症.
在PLWH中肠道微生物群和炎症性细胞因子之间的关系与对抗逆转录病毒治疗 (ART) 的不同反应仍然不清楚.
了解这些关联对于优化艾滋病治疗策略至关重要.

研究的目的:

调查肠道微生物群组成和PLWH中的炎症性细胞因子之间的关联.
探索免疫反应者 (IRs) 和不响应者 (INRs) 之间的肠道微生物特征的差异.
确定与免疫状态和ART疗效相关的潜在微生物生物标志物.

主要方法:

招募了68个PLWH (35个IR,33个INR) 和27个健康对照 (HC).
收集血液和便样本进行分析.
使用Luminex技术量化血微生物转位生物标志物和炎症性细胞因子.
通过便样本的细菌16S rDNA测序分析了肠道微生物组成.

主要成果:

与HC相比,HIV组的肠道微生物丰度发生了变化,Fusobacteria和Actinobacteria增加,Ruminococcaceae和Faecalibacterium减少.
与IR相比,INRs表现出不同的微生物特征,其中Actinomycetales较高,Sutterellaceae较低.
瘤亡因子-α (TNF-ɑ) 和CD54水平与特定的细菌丰度 (例如,Dialister) 有负相关.
CD4+ T细胞计数与特定的肠道细菌,如Butyricimonas和Veillonella有关.

结论:

肠道微生物失调症可能会导致不同免疫反应和PLWH中ART的治疗效果.
特定的微生物变化与免疫反应状态和炎症标志物有关.
对肠道微生物群调节的进一步研究可能为改善ART结果提供新的治疗途径.

关键词:

我们的肠道微生物群.艾滋病病毒艾滋病病毒艾滋病病毒免疫学非响应者.免疫反应者免疫反应者炎症性细胞因子是炎症性细胞因子.

更多相关视频

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

Published on: May 2, 2017

An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions

An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions

Published on: June 30, 2021

相关概念视频

Factors Affecting the Risk of Infection

Factors Affecting the Risk of Infection

The hosts' susceptibility to infection depends on several factors. The integrity of the skin and mucous membranes helps protect the body against microbial attacks. When the skin is altered, the chance of infection, limb loss, and even death increases.
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin...

Anatomy of the Intestines

Anatomy of the Intestines

Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...