在结直肠癌中功能替代背接事件的转录组规模分析
Qianqian Ning1,2,3, Qian Jin1,2,3, Lei Zhao4
1Cancer Institute, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, 221004, Jiangsu, China.
Journal of translational medicine
|April 24, 2025
概括
替代背接 (ABS) 事件在结直肠癌 (CRC) 中很普遍. 异常表达的ABS circRNA与CRC瘤发生和患者存活有关,提供潜在的治疗点.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 癌症研究 癌症研究
背景情况:
- 循环RNAs (circRNAs) 是通过基因逆向拼接形成的.
- 替代背接 (ABS) 可以从单个基因中生成多个circRNA.
- ABS事件在结直肠癌 (CRC) 致癌的作用在很大程度上是未知的.
研究的目的:
- 描述CRC中ABS事件的情况.
- 为了确定与CRC致癌症和患者预后相关的ABS事件.
- 研究特定的ABS环RNA在CRC中的功能作用.
主要方法:
- 来自176个CRC样本的转录组测序数据的分析.
- 在瘤和正常组织之间识别差异表达的ABS circRNAs.
- 对预后性ABS事件进行考克斯回归分析.
- 对于circXPO1变异的体外和体外功能测定.
主要成果:
- 确定了19,611个高可信度circRNAs,其中91.1%是复发性.
- ABS circRNAs占所有已识别的 circRNAs 的68.8%,这表明其流行程度.
- 552个ABS环RNA被异常表达,并与癌症特征相关.
- 13个ABS circRNAs作为CRC存活的独立预后指标.
- 与circXPO1-1.不同的是,circXPO1-2抑制了CRC细胞的增殖,迁移和入侵,而circXPO1-1.
结论:
- 在CRC转录组中,ABS事件普遍存在.
- 异常表达的ABS circRNA与CRC瘤发生密切相关.
- ABS circRNAs代表了对CRC具有临床益处的潜在新型治疗点.
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