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在植物中,通过共同表达CRISPR/Cas和发育调节剂来促进类植物的基因组编辑

Gilor Kelly¹, Elena Plesser¹, Eyal Bdolach¹

¹Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Rishon LeZion, 7505101, Israel.

The Plant journal : for cell and molecular biology

|April 25, 2025

概括

我们开发了一种在中植物基因组编辑 (IPGEC) 系统,以更快地进行品种. 这种方法可以在土壤中生长的植物中进行直接的基因组编辑,绕过了漫长的组织培养和体克隆变异问题.

科学领域:

植物生物技术植物生物技术
基因组学就是基因组学.
园艺科学园艺科学

背景情况:

传统的类基因组编辑依赖于耗时的组织培养,导致延迟和索马克隆变异.
开发高效,直接的基因组编辑方法对于加速类繁殖计划至关重要.

研究的目的:

引入和验证植物基因组编辑系统 (IPGEC),用于在土壤中种植的类植物中进行直接转化和再生.
评估IPGEC在产生编辑类植物和获得无转基因个体方面的效率.

主要方法:

通过Agrobacterium tumefaciens开发了IPGEC系统,用于通过基因组编辑,射击诱导和T-DNA传递组件的短暂共同表达.
利用单导向RNAs (sgRNAs) 准PHYTOENE DESATURASE (CsPDS) 基因以诱导突变.
使用高通量选来识别无转基因,基因组编辑的植物.

主要成果:

通过IPGEC系统,在编辑的树组织中成功诱导了de novo发芽再生.
通过准CsPDS基因,生成突变的白化种子,证实了同卵性/双性编辑植物的产生.
证明了使用这种方法获得无转基因基因编辑类植物的潜力.

结论:

在中植物基因组编辑 (IPGEC) 系统为品种的传统组织培养方法提供了更有效的替代方案.
需要对发育调节器进行培养特异性优化,以最大限度地提高转化和再生率.
这项技术有可能通过加快改进类品种的发展,彻底改变果树育种.

关键词:

红宝石 (ruby) 是一种红宝石.类的类植物.在 de novo 介质系统的诱导过程中.基因组编辑基因组编辑在植物中的植物.技术提前技术提前转化转化转化转化转化转化转化转化转基因植物是转基因植物.

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