在CAST/EiJ小鼠中,疫苗病毒和mopox病毒感染的比较致病性:探索腹大病和转录组形状
Yongzhi Hou1, Jianrong Ma1, Baoying Huang2
1NHC Key Laboratory of Human Disease Comparative Medicine, National Center of Technology Innovation for Animal Model, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Animal models and experimental medicine
|April 25, 2025
概括
在CAST/EiJ小鼠中,疫苗病毒 (VACV) 和mpox病毒 (MPXV) 感染的结果不同. MPXV和低剂量的VACV会导致腹壮大,而高剂量的VACV会导致快速死亡,并确定了共享和独特的分子通路.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 疫苗病毒 (VACV) 和mpox病毒 (MPXV) 是密切相关的正体病毒.
- CAST/EiJ小鼠是 ортопокс病毒研究的常见模型.
- 在CAST/EiJ小鼠中MPXV和VACV感染的细节性病理学和分子特征尚不清楚.
研究的目的:
- 为了比较MPXV和VACV在CAST/EiJ小鼠中的致病性.
- 为了阐明脊柱病理学和分子机制的orthopoxvirus诱导的大病.
- 为了分析脏基因表达特征,以应对不同的骨质疏松病毒感染.
主要方法:
- 通过腹膜内注射,CAST/EiJ小鼠感染了不同剂量的VACV或MPXV.
- 监测疾病进展,体重,病毒载量和标位.
- 脏组织使用组织病理学,免疫组织化学和RNA测序来分析.
主要成果:
- 低剂量VACV (VACV-L) 和MPXV诱导的脊髓巨变,其特征是外骨质造血和巨透.
- 高剂量的VACV (VACV-H) 导致了快速致命性,脏亡,拥堵和出血.
- 转录组分析显示了VACV-L和VACV-H的独特基因表达模式,但类似的途径 (,MAPK,PI3K-Akt) 与VACV-L和MPXV感染中的大病有关.
结论:
- 这项研究系统地比较了CAST/EiJ小鼠的VACV和MPXV病原性.
- 脊髓转录组分析提供了对正病毒诱导的脊髓大病机制的见解.
- 不同的病毒剂量和类型在小鼠脏中引起不同的病理结果和分子反应.
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