在印度尼西亚传播和表型特征ESBL产生的大肠杆菌在印度尼西亚

在印度尼西亚传播和表型特征ESBL产生的大肠杆菌在印度尼西亚

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Tati Ariyanti¹, Suhaemi Suhaemi¹, Sri Mulyati¹

¹Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Bogor, Indonesia.

Open veterinary journal

|April 25, 2025

概括

生产扩展谱β-乳糖酶 (ESBL) 的大肠杆菌,在动物中越来越大的威胁,显示出对常见抗生素的高耐药性. blaCTX-M基因是最常见的,这表明需要紧急控制策略.

科学领域:

兽医微生物学兽医微生物学
抗微生物耐药性抗微生物耐药性
公共卫生公共卫生

背景情况:

在动物和人类中,由扩展光谱β-乳糖酶 (ESBL) 产生的大肠杆菌感染的增加.
抗生素耐药性的升级构成了严重的公共卫生威胁.
了解与动物相关的大肠杆菌的流行和耐药性模式对于控制至关重要.

研究的目的:

分析ESBL产生大肠杆菌中的blaCTX-M,blaSHV和blaTEM基因的表达.
评估这些分离物的抗生素耐药性概况.
为了研究来自兽医科学研究中心和国家研究与创新机构的分离物.

主要方法:

从兽医样本中识别大肠杆菌分离物,使用MacConkey agar与cefotaxime.
通过聚合酶链反应 (PCR) 检测blaCTX-M,blaSHV和blaTEM基因.
根据CLSI标准,使用抗生素盘进行抗菌敏感性测试.

主要成果:

产生ESBL的大肠杆菌的患病率在肉,蛋和鱼肠中为16.5%.
blaCTX-M是最常见的基因 (93.75%),其次是blaTEM (81.25%) 和blaSHV (25%).
所有分离物体都表现出多药性耐药性,对安培和硫甲醇的耐药性为100%,仅对氨基醇有敏感性.

结论:

在产生ESBL的大肠杆菌分离物中,blaCTX-M基因占主导地位.
这些细菌对安培和硫甲醇表现出完全的耐药性.
这些分离物表现出显著的多药性耐药性,突出显示了兽医和公共卫生领域的关键挑战.

关键词:

这就是ESBL.埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.蓝色TEM的时间在blaCTX-M中使用.blaSHVV 的意思是

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