单亲细胞和多亲细胞芽质体的起源和发展
Yan Zhao1,2, Andrea Fernández-Montoro3, Greet Peeters1
1Laboratory for Cytogenetics and Genome Research, Department of Human Genetics, KU Leuven, 3000 Leuven, Belgium.
iScience
|April 25, 2025
概括
早期胚胎的全基因组异常可能导致发育问题. 牛头原体的哈普洛型查揭示了异常细胞的应激反应,影响了发育,并表明了改善体外受精结果.
科学领域:
- 生殖生物学 生殖生物学
- 发育生物学是发展生物学.
- 遗传学 遗传学 是一个
背景情况:
- 全基因组 (WG) 异常,包括单亲双胞胎和三亲双胞胎,是胚胎致死性的重要原因,偶尔会导致与双亲双胞胎细胞共存的活产.
- 了解WG异常胚芽细胞的早期发育对于阐明各种复杂的遗传条件的形成至关重要,如雄原体,雌原体,三重体,嵌合体和混合体.
研究的目的:
- 在早期牛胚胎发育过程中调查全基因组异常芽细胞的起源和发育轨迹.
- 确定WG异常细胞中发育障碍背后的细胞机制.
主要方法:
- 从最初的裂变阶段对118个牛胚芽细胞的哈普洛型分析.
- 培养全能芽细胞的培养到三个植入前阶段.
- 对446个细胞的转录组分析,以评估基因表达和细胞反应.
主要成果:
- 在多极性和双极性裂变异构体中确定了异构的分裂,导致父母基因组分离成不同的胚芽细胞.
- WG异常细胞表现出显著的应激反应,导致发育障碍,其特征是细胞循环停止或不能形成芽细胞.
- 这些异常细胞在植入前胚胎中的患病率代表了在异常胚胎发育中以前被低估的因素.
结论:
- 异质分裂是早期胚胎中产生细胞多样性的关键机制,包括WG异常.
- 应激反应对于调解WG异常细胞的发育命运至关重要,导致停止或异常芽细胞形成.
- 基于哈普洛型的查有可能通过识别和潜在地减轻WG异常的影响来改善体外受精的结果.
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